Screening Tumor Antigens Of Nasopharyngeal Carcinoma By Serological Proteomics Technologies

Objective: Tumors express specific antigens, which are targets of a humoralimmune response. Such specific tumor-associated antigens are of increasingimportance for diagnosis and immunotherapy. Nasopharyngeal carcinoma israre in most populations around the world but common in southern China. Ithas been suggested that Epstein-Barr virus infection may be a major riskfactor for nasopharyngeal carcinoma, and a variety of methods have beenused to detect antibodies against EBV antigens in patients with thisdisease. But researches for nasopharyngeal carcinoma cell proteinsare few. Here, we used serological proteomics technologies to screentumor antigens of nasopharyngeal carcinoma.Methods: The nasopharyngeal carcinoma cell line CNE-1 was used as asource of tumor cell proteins for Western blot analysis in which individualsera with special immunoreaction were selected and for immunoprecipitationanalysis. Then, differential proteins are analyzed by mass spectrometry andbioinformatics methods.Results: Membrane-bound Ig M was identified in the differential proteinband.Conclusion: It may be a convenient, simple and sensitive way to screentumor antigens by proteomics technologies.