Study On Anti-aging Effect Of Ginsenosides On Skin Of The Aging Model Mice And HaCaT Keratinocytes

Aim To study the anti-aging effect of Ginsenoside on the skin in vitro and vivo. In vitro is to investgate the survival ratio and the damage level of HaCaT cells following ultraviolet B (UVB) radiation at various dosages,and observe the protective effect of Ginsenoside Rg₁ and Rb₁ on growth behavior of the cell subjected to UVB.In vivo is to investigate the anti-aging effect of Ginsenosides on skin of mice. Methods MTT assay was employed to analyze the cell survival ratio after UVB radition of 30,60,90,120mJ/cm².The damage of nucleolus and the protective effect of Ginsenoside Rg₁ and Rb₁ with a dose of 20μg/ml were scanned by Hoechst33258 staining and single cell gelelectrophoresis assay(SCGE). Mice were randomzed into four groups rnomal control,aging model without treatment of Ginsenosides,aging model with treatment of Ginsenosides 50mg/kg.d^-1, aging model with treatment of Ginsenosides of 100mg/kg.d^-1. The aging mice model was made by subcutaneous administration in cervix with a dose ofD-galactose(1000mg/kg)for 42 days. At the same time, the aging mice take GinsenosidesCSOmg/kg.d^or lOOmg/kg.d"1) orally. After 42 days,mice were put to death by take blood sample by pulling out eyeball and detect the activities of Catalase(CAT) and Glutathione peroxidase(GSH-Px) in whole blood within 48 hour.Denude the skin of cervix with sulfureted hydrogen two days before the mice being put to death.Chose the skin of cervix,Scaped the hypodermal fat and bindweb and frosted the skin tissue.The activity of Superoxide Dismutase(SOD) in skin, the amounts of Maleic Dialdehyde(MDA) and hydroxyproline in skin were checked after 42 days. Results It was found that the cell survival ratio decreased gradually and the damage of nucleolus aggravated as the radiation dose increased from 30 mJ/cm2 to 120mJ/cm2. Protective effect of Ginsenoside Rgi and Rbj can be observed against UVB radiation-induced HaCaT cells growth inhibition and nucleolus damage.But with a fixed dose of radiation, Ginsenoside Rgi and Rbi of 20ng/ml play different protective effect. Ginsenoside Rgi of 20ng/ml obviously increased survival ratio under 30,60 mJ/cm2 UVB radiation and shortened the tail length of the HaCaT cells under 30 mJ/cm2 UVB radiation and decreased the number of apoptosis HaCaT cell under 30 mJ/cm2 UVB radiation. Ginsenoside Rbj of 20\ig/m\ significently increased survival ratio under 30,60,90,120 mJ/cm2 UVB radiation and shortened the tail length of the HaCaT cells under 30,60,120 mJ/cm2 UVB radiation and decreased the number of apoptosis HaCaT cell under 30 mJ/cm2 UVB radiation. Ginsenosides of lOOmg/kg.d'1 can significantly increase activities of CAT and GSH-Px in whole blood and SOD in skin tissue and decrease the amount of MDA in skin tissue. Ginsenosides of SOmg/kg.d"1 can statisticallyimprove the amount of hydroxyproline in skin tissue of aging model mice(p<0.05) and Ginsenosides of lOOmg/kg.d'1 improved it more greatly(p<0.01). Conclusion In vitro UVB radiation inhibit HaCaT human keratinocytes growth and Ginsenoside Rgi and Rbi can antagonize the effect,and in vivo Ginsenoside of lOOmg/kg.d"1 has remarkable anti-aging effect in skin on model of aging mice induced by D-galactose.