Research On Spectrum Characteristic Of Hypoxia Stress Peptide And Its Expression After Trauma

Objective & Background: Stress is the common physiological characteristic of the organism. Stress response, as a ubiquitous biological phenomenon, is able to be induced by many stress factors such as hypoxia, trauma, heat and so on. Some proteins, which are conservative SRP including Heat Shock Protein family,are synthesized after stress response. Hypoxia Stress Peptide is a new SRP which is studied and discovered in prophase time. A great deal of HSP was detected in serum of the patients who got high altitude sickness. By NMR spectroscopy analysis, the special dactyl gram zone exists in HSP molecule. There has no report on the changes of the expression of HSP in serum of injured patients and on the significance of these changes. The expression level of HSP is still not clear in serum after healthy people ascending Tibet plateau. The ultraviolet absorptive spectrum of HSP is still not analyzed. And so, those were respectively studied including the changes of the expression of HSP in serum of patients after encephalic injury, the changes of expression of HSP in serum of rabbits after bile duct is injured by hemostat and the significance or factors of these changes in order to get the evidence of HSP's usages in the diagnosis, therapy and prognosis of trauma. Method: Serums of patients were collected in 48hours and kept in -40℃after encephalic injury. Adoptable standards of the injured group were ISS≥16,all pateints≥18years old and all patients were survived. The control group objects were all healthy people. The model of wounded rabbit's bile duct by hemostat was established. The interfering factors included hemostat force and duration. All rabbits were distributed randomly into 14 groups (5rabbits per group). The serums of all injured objects were collected and kept in -40℃after 3days, 7days, 14days and 30days. The serums of healthy young people, who includes the plain group (n=30), the plateau 3d group (n=30), the immigrant group (n=30) and the native group (n=30), were collected and kept in -40℃. The quickly chemical method was used to determine HSP of all serums. The RIA method was used to determine cortisol. The Beckman-Coulter automatic biochemical analyzer was used to determine biochemical indexes. The 13C-NMR spectroscopy was used to analyze HSP's structure and the ultraviolet spectrophotometer was used to detected ultraviolet absorptive spectrum of HSP. Result: 1)After encephalic injury, the HSP or cortisol level of the injured group is higher significantly than that of the control group (HSP :101.8±22.4mg/l,C:300±160ng/ml,N=48,p<0.01).The HSP level shows the trend as follows: expression higher after injury early, the highest level at 24hours after injury and fall gradually. 2) After the rabbit's bile duct was wounded by various degrees hemostat, theexpression levels of HSP, TBIL, DBIL, ALT, AST and GGT were higher significantly than that of the control group (p<0.05). And the hemostat force or duration is stronger or longer, the expression levels are higher. 3) After ascending Tibet plateau, the levels of HSP, CK, LDH and HBDH show a more significant increase in the 3days group than that in the plain group(p<0.01), but not so high in the 1 year group whose expression levels show a more significant decrease than that in the 3days group(p<0.01). 4) The largest ultraviolet absorption wavelength is 204.88nm. By the 13C-NMR spectroscopy of HSP, there may be conclude amino acids as follows: Val,Ile,Trp,Phe,Tyr,Arg,Lys,Pro and Thr. Conclusion: 1) After encephalic injury early, the HSP or cortisol level of the injured group shows a significant increase. And so, HSP can be used to indicate stress response degree when organism gets acute injury. 2) After the rabbit's bile duct was wounded by hemostat, the expression level of HSP shows dosage effect correlation with the hemostat force or duration. And the hemostat force or duration is stronger or longer, the expression levels of HSP and hepatic biochemical indexes are higher. By detecting the changes of them, it is observed that the rabbit's bile duct is wounded stronger or not. 3) After the plain people are exposed the high altitude hypoxemic environment early, HSP and myocardial enzyme show a significant increase. After one year in plateau, the cells get acclimation gradually and the expression degrees of HSP or myocardial enzyme decrease step by step. HSP can protect body cellsand decrease hypoxemic injury. 4) HSP can be inducted and synthesized not only by hypoxia but also by trauma or other stress factors. Many technical means make these clear as follows: the ultraviolet absorption spectrum characteristic and some molecule framework information of HSP. On the basis of these studies, the framework and property of HSP will be investigated and get clear.