The Experimental Study On Basic Fibroblast Growth Factor Combined With Autogenous Inside-out Vein Graft For Repairing Facial Nerve Defect In The Rabbit

Objective This study describes a modification of the vein graft technique inside-out vein graft combined with basic fibroblast growth factor, creates an autogenous vein conduct with the collagen-rich, Schwann cells and injected bFGF exposed to regeneration axons. To explore the effect of autogenous inside-out vein graft conduit combined with bFGF on peripheral nerve regeneration. Methods 24 adult white rabbits were selected and divided into four groups. The facial nerve of rabbit in the left side was severed and bridged by autogenous inside-out vein graft as group C and D while it was severed and bridged by autogenous standard vein graft as group A and B. 0.3ml bFGF solution (0.75ug/ml) was intravenously injected to the vein graft conduit as group B and D, the same amount of saline solution as group A and C. Microscopic examination and nerve conduct velocity were performed at 100th day after operation. Results The nerve fibers were grown into vein graft conduct in all groups at 100^th day after operation. At 100^th day regeneration nerve on the inside-out vein graft combined with bFGF side showed superior function results-faster conduction veiocities(p<0.05)and distinct histological results compared with the others. Bridging a defect in facial nerve with inside-out vein graft combined with bFGF has the best effect in all groups. Conclusion The adventital surface of vein promotes nerve regeneration by an environment with collagen-rich and Schwann cells. Injecting bFGF improves the environment further. Inside-out vein graft combined with bFGF is an ideal nerve bridge for treatment of nerve defect and its effect is certain.