Preparation Of Maca Mulatta Model Of Resuscitation After Selective Cerebral Ultra-deep Hypothermic Blood Flow Occlusion

Objective: To study the modal preparation of monkey of resuscitation after selective cerebral ultra-deep hypothermia and blood flow occlusion,then specificate the model.Methods:Ten 4-10 year-aged maca mulattas were randomly divided into three groups, four-vessel occlusion group, two-vessel occlusion group and identical temperature perfusion group. Incising the sheathes of bilateral carotid arteries, ligating right common carotid artery, a catheter was distally inserted into right internal jugular artery (ICA) to connect cold prefusion system, two catheters were distally and proximally inserted into right internal jugular vein(IJV) and right femoral vein(FV) to connect the lead-in of the device of ultrafiltration and rewarming, a catheter was proximally inserted into left femoral vein to connect the outfa of the device of ultrafiltration and rewarming. a catheter was proximally inserted into right IJV to monitor central venous pressure(CVP). Clamping bilateral exteral jugular veins(EJVs), left ICA and IJV, four-vessel occlusion group was also clamped bivertebral artery (VA). Cold solution (4.0 + 0.5℃) was distally perfused into the right ICA to cool the brain, at the same time, two catheters were distally and proximally inserted into right IJV and right FV to reflu the blood of veins, the solution of refluing was perfused the systemic circulation by left FV after ultrafiltration and rewarming. The temperature of brain was maintained 14.3~16℃ for 60min. MRI was examinated before and after operation,EEG,the vital signs and the hemodynamical parameters were monitored during the experiment, the dysfunctional scales of neurology were done every week, the brains and other vital organs were examined by microscopy.Results:In all of ten monkeys, the hemodynamical parameters of two-vessel occulation were steady during the operation, and all of them lived up for ever after filled 60 minutes. MRI was normal after operation. EEG was normal and the functions of neurological deficient scale were normal after operation. There were no morphological abnormities in all vital organs. Theothers of identical temperature perfusion group and four-vessel occlusion group were not resuscitation after filled 60 minutes and died, and there were different morphological abnormities in all vital organs. Conclusion: It is safety that monkey resuscitate from selective cerebral ultra-deep hypothermia and blood flow occlusion of bilateral common carotid artery for 60 minutes and it is normal to the hemodynamica] parameters, brain and nervous function.