Impact Of Rat Anterior Pituitary Cells Exposed To Nonylphenol

Objective To study the impact of primary rat anterior pituitarycells exposed to NP, and provide the available information of the impact onthe creature exposed to endocrine disrupting chemicals-NP. Methods 1. Primary rat anterior pituitary cells are dispersed and cultured invitro. 2. Rat anterior pituitary cells are identified by observing the cellsappearance characteristics , by the expression of LH protein in the cellsdetected by immunocytochemistry and by secreting LH of the cellsdetected by radioimmunoassay. 3. The proliferation of the cells were detected by MTT assay; thedistribution of the cells were analysed by FCM; the expression of PCNAprotein in the cells were detected by immunocytochemistry. 4. The expression of ER protein in the cells are detected byimmunocytochemistry. Result 1. The dispersed and cultured cells show the appearance characteristicsand unique function of anterior pituitary cells, according to the results ofmicroscopical observation, immunocytochemistry and radioimmunoassay. 2. It shows that the NP has dose-response relationship with the Avalue of MTT assay, the PI of FCM and the PCNA positive average lightdensity, respectively. Compared with the negative control group, the PCNApositive average light density of the cells exposed to 1×10-6 mol/L NP aresignificantly decreased. 3. Compared with the negative control group, the ER positive averagelight density of the cells exposed to 1×10-9 mol/L,1×10-8 mol/L,1×10-7mol/L,1×10-6 mol/L NP are significantly increased. It does not showdose-response relationship between the NP concentration and the ERpositive average light density. But there are significantly differences inevery dose group of NP. Conclusion 1. Rat anterior pituitary cells are cultured successfully in vitro, bydispersing,culturing and identifying the anterior pituitary cells from ratpituitary. 2. NP inhibits the cells proliferation by preventing the expression ofPCNA in the cells. It shows dose-response relationship that NPconcentration enhanced and the cells proliferation inhibited. NP interferesnormal proliferation of the cells. 3. It is not confirmed that NP induces the apoptosis of the cells. 4. It shows the endocrine interfering mechanism of NP binding ERthat NP adduces the ER expression increased.