Protection Of Influenza Virus Inactivated Vaccine And DNA Vaccine In Diabetic Mice

Plasma glucose was examined after various doses injections of streptozotocin (STZ) in BALB/c and KUNMING mice. In KUNMING strain, the hyperglycemia was less severe in females than in males. In BALB/c mice, males became hyperglycemic after intraperitoneal injection of a single high dose or multiple low dose of STZ, however, females could not be induced to diabetes. These results suggested that host factors such as strain and sex apparently influence both the development and expression of STZ-induced diabetes. We found that a stable and persist model of type I diabetes could be established by a single injection with STZ at a high dose in male BALB/c mice. The pathological states of the diabetic mice maintained during the whole study.Adult BALB/c mice, both healthy and diabetic, were immunized once or twice with monovalent inactivated influenza virus vaccine, and their serum specific IgG antibodies were detected by enzyme-linked immunosorbent assay (ELISA). The mice were challenged with influenza virus at a lethal dose, and the protective effect of vaccine was observed to provide reference for clinical studies.