| The abuse of opiates is still a growing public health problem in the world. Opioid is characterized for its abuse potentiality while cessation of using it results in the emergence of these drug-opposite responses as the withdrawal syndrome which can inevitably lead to continue drug use in dependent users. Opioid abuse consequently leads to health and social problems. Many countries make laws to fight against the drug deals, and substantial amount of the scientists in the world devote themselves to find new strategies to prevent and treat drug addiction.Recently, much progress has made in the treatment programs with the discovery on opioid receptors and a series of agonists and antagonists. Three main programs have been made such as cold turkey, drug detoxification and non-drug treatment. And the treatment programs are inadequate for many reasons such as serious abstinent syndromes, addictive potential or a too long period of treatment. So, to find out an efficient remedy is the dreams of the human.Gao and Liu designed a series of somatostatin analogues by replacement of several amino acids from a kind of somatostatin analogue. And that, these SOMas had ever been demonstrated to reduce morphine-induced dependence and naloxone-precipitated withdrawal symptoms. It is promising to be used in the treatment or prevention of opiate abuse in the future. In this research, we will further investigate their selectivity of opioid receptors in order to know better of the mechanism involving in.The first part of this research was focused on investigating the binding characteristics of the receptors by radioligand binding assay. The radioligand binding assay was carried out on Chinese hamaster ovarian cells, transfected with mu, delta and kappa-opioid receptor respectively, to determine if the SOMa-lys and SOMa-arg selectively bind opioid receptors with a non-selective antagonist 3H-diprenorphine. We isolated the membrane proteins from cultured CHO cells, and then put it in the same...
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