| Platelet is the smallest of blood cells, being only fragments of megakaryocyte cytoplasm, yet they have a critical role in normal haemostasis and are important contributors to thrombotic disorders. In clinical, platelet demands are increasing with the development of medical technology. But using platelet products have many drawbacks, such as diseases rooted in blood, immunoreaction. Efforts to overcome these shortcomings have resulted in an array of novel platelet products and substitutes. This paper was divided into four parts.The first part, is to isolate, purify and identify platelet membrane glycoprotein. Platelet was isolated by differential centrifugation; 1%TritonX-100 was used to dissolve platelet membrane glycoprotein; wheat germ agglutinin affinity chromatography was applied to purify glycoprotein. Platelet membrane glycoprotein was obtained, which provided the foundation for more study. The second part, activity was determined by means of platelet aggregation. the result showed that the obtained glycoprotein could inhibit ristocetin-induced and ADP-induced platelet aggregation. In the third part, Reverse phase evaporation method was used to prepare glycoprotein-liposomes. The composition of the glycoprotein-liposomes were lecithin/cholesterol (ratio 4:1), glycoprotein/lipid(ratio 1:100).Glycoprotein-liposomes have -0.3~-1mv electric charge, diameter of Glycoprotein-liposomes was 266 ± 23nm(n=10) and entrapment efficiency was 75.4%±8.2%(n=6). Well-proportioned particle glycoprotein-liposomes could be obtained by Using reverse phase evaporation. In The fourth part, activity was characterized by means of Platelet aggregation. The result showed that The glycoprotein-liposomes could promoted ristocetin-induced platelet aggregation, which best concentration was 140μg/ml.
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