| The coronary heart disease patient that the pulse progressing from stability angina pectoris for acute coronary syndrome which important pathological mechanism physiology mechanism is speckle piece instability , blood platelet activation , thrombopoiesis .Only activating platelet can have biological behaviors such as adhesiveness,deformation,aggregation and release.Compared with silent platelet,activating platelet has changed significantly in membrane glycoproteins,especially changes in PAC-1 and CD62P. PAC-1 is fibrinogens receptor also call it GPIIb/IIIa receptor. Every kind of stimulus such as 5-HT,ADP,thrombin enzyme et al combines to its receptor must through the combination process of GPIIb/IIIa and fibrinogen.It is the final pathway of platelet aggregation. CD62P is the glycoprotein of platelet and grainmembrane inside the platelet.During the process of platelet activation these membrane glycoprotein will take place conformation change or quantity rearrangement, participate the process of platelet adhere to endothelium, platelet adhere to platelet and platelet adhere to leukocyte.CD62P is one of the marks of platelet activation because the amount of CD62P on activating platelet membrane could add up to more than 15 times.Objective Adopt the FACSCalibur of USA Becton-Dickinson company flow cytometry film glycoprotein CD62P and the PAC-1 expressing from activation blood platelet are horizontal start , discuss if coronary heart disease patient body inner blood platelet be in altitude activation state; If blood platelet activation degree increase weight but increase by with coronary artery lesion degree. If CD62p , PAC-1 can assume state of illness rotating return to the index observing with coronary heart disease medication and the curative effect. Reveal CD62P and the horizontal PAC-1 change relation with coronary heart disease, hyperglycaemia and lipid metabolic disorder, first step investigation and discussion it's the correlativity with sexual, interferes with to look forward to reaching the early phase being in progress to the disease , the course keeping an atherosclerosis within limits having effect, the occurrence cutting down the cardiovascular event , the quality of life improving and improving a patient.Methods Choose 82 patients with the coronary heart disease , 22 patients with acute myocardial infarction (group AMI), 40 patients with unstable angina pectoris (group UAP), 20 patients with stable angina pectoris (group SAP), and 20 controls which through coronary angiography or the heart 64 row of helix CT verification there be no the lesion patient being the regular collation group. Express making use of the flow cytometry skill to check when being hospitalized and going through front CD62P and PAC-1 curing 14 day's or leaving hospital , parallel it's horizontal changed. Determine indices such as blood sugar , blood grease on analytical full-automatic biochemistry instrument.Results four group of crowd common material comparion: Patient's age , sex compose , blood pressure , platelet count and parallel weight exponent difference have no notable significance.1,Crowd (the AMI group , UAP group , SAP form and contrast a group) cures front CD62p value four set being respectively 15.95±3.97,13.89±4.68,6.59±1.49,3.36±1.07, parallel difference of four set of equal room numbers has notable meaning (P value <0.05) , difference has notable meaning set liang comparatively without exception (P value <0.05); Crowd cures front PAC-1 value four set being respectively 15.49±3.20,13.77±3.53,7.03±1.33,3.61±1.27, parallel difference of four set of equal room numbers has notable meaning (P value <0.05) , difference has notable meaning set liang comparatively without exception (P value <0.05). Coronary heart disease cures queen CD62p set , PAC-1 value is respectively (8.00±2.44,7.08±2.35,3.58±1.37),(8.43±1.88,6.89±2.19,3.33±1.72),curing parallel difference of front having notable meaning , (P value <0.01). curing the queen AMI group , UAP group CD62p , PAC-1 and contrasting parallel difference of group having notable meaning (P value <0.01).2,Coronary heart disease patient CD62p , PAC-1 are parallel according to coronary artery lesion degree . Three lesion group and CD62p putting the lesion group off pair express (13.82±4.63,13.78±5.24vs10.75±5.20)without exception higher than monobranch lesion group , comparatively same difference of equal number of group has notable meaning (P value <0.05).Three lesion group and PAC-1 putting the lesion group off pair express (14.46±4.62,12.82±3.69vs10.46±3.62)also without exception higher than monobranch lesion group , comparatively same difference of equal number of group has notable meaning (P value <0.05). Three lesion group do not have notable significance with CD62p putting the lesion group off pair , parallel difference of equal number of PAC-1 group (P value> 0.05). Every group CD62p , PAC-1 curing the queen are without exception higher than regular collation group,AMI forms , UAP forms comparing with contrasting a group difference to have notable meaning (P value <0.05).3,Appearance the men and women coronary heart disease patient is cured there being no notable significance difference around CD62p , PAC-1 (P value <0.05)4,Four crowd of group (the AMI group , UAP group , SAP form and contrast a group's) on an empty stomach blood sugar with state of illness increase weight but rise gradually to be respectively: 6.61±1.69 mmol/l,6.46±2.58 mmol/l,6.37±3.33 mmol/l,4.97±1.08 mmol/l, AMI forms , UAP forms with contrasting parallel equal group number difference having notable meaning (P value <0.05);Crowd's four set of general cholesterin are horizontal be respectively: 4.19±1.47 mmol/l,4.07±0.99 mmol/l,4.09±1.37 mmol/l,2.83±1.11 mmol/l, parallel difference of equal numbers of group have notable meaning (P value <0.01). LDL-C value rises gradually with the coronary heart disease progressing , (2.73±0.68 mmol/l vs2.15±0.88 mmol/l) difference has notable meaning but only when the AMI group compares with to contrasting a group(P value <0.05).Dangerous coronary heart disease patient factor blood sugar and the blood platelet activation index PAC-1 and CD62p thickness has the correlational dependence(r=0.302 and 0.205,P value<0.05);the LDL-C density also has correlational dependence with blood platelet activation index PAC-1 and CD62p(r=0.212 and 0.205,P value<0.05).Conclusion 1. CD62P and PAC-1 are sensitive blood platelet activation radioactivation index which is a good monitoring that the blood platelet cures as resisting index. Coronary heart disease every equal group existence blood platelet activation phenomenon , CD62P , PAC-1 are expressed overtoping the collation group without exception. Among them, AMI patient blood platelet activation degree is the heaviest , secondly, is an UAP group , then is a SAP group and contrasts a group in time. Every group CD62p , PAC-1 curing the queen are still higher than regular collation group.2. Range (count) is divided into group according to coronary artery lesion,three lesion group CD62p and PAC-1 putting the lesion group off pair express without exception higher than monobranch lesion group , comparatively same difference of equal number of group has notable meaning. Three lesion group do not have notable significance with the lesion group off pair .3. The men and women coronary heart disease patient is cured there being no notable significance difference.4. The patient blood sugar , LDL-C having trend rising gradually with the fact that lesion degree increasing weight , which with the CD62p , PAC-1 has rectify correlational dependence.
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