A Study On The Development Pathological Changes Of Reperfusion Injury And Its Correlation To The Expressions Of Gelatinase,TIMP-1,GFAP And PCNA In Cerebral Tissues Of Rat After Focal Cerebral Ischemia

Purpose To investigate the development pathological changes of reperfusion injury and its correlation to the expressions of gelatinase, TIMP-1, GFAP and PCNA in cerebral tissues of rat after focal cerebral ischemia Methods 2 hours right intraluminal middle cerebral artery occlusion with 3h-7dreperfusion were induced in 36 SD rats, accompanying with sham operation contral in 7 rats . The development pathological changes of specimens of the brain after3h-7d reperfusion were observed by HE and Tunel stained,the expressions of gelatinase, TIMP-K GFAP and PCNA in cerebral tissues of rats were detected by immunohistochemistry method after focal cerebral ischemia with different reperfusion period.Results there was significant difference in the lesion of cerebral tissue between the groups of local cerebral ischemia with reperfusion and sham operartion groups in rats, P<0.01; vasogenic edema can be observed in the course of reperfusion, peaked at 24h, 72 h respectively ,and at 24h accompanied with focus cerebral hemorrhage.In the core zone of ischemia cerebral tissue, necrosis cells can be observed easily and from 24h to 7d accompanied with inflammation cells infiltration; the hyperplasia of astrocyte and repairment of cerebral tissue began at 72 h after reperfusion ,but apoptosis cells could be observed all the time especially in perilesional (penumbra).There was positive relevant between apoptosis cells and cerebral edema, there were...