Protective Effect Of LW Against Cerebral Ischemia Injury

Objective To study the protective effects of LW on cerebral ischemia injury and itsmechanism.Methods (1) The experiments of quick decapitation and anoxia under normal pressure model in mice was used to study the effects of LW on acute cerebral ischemia and anoxia. (2) The focal cerebral ischemia reperfusion modal was made to observe the effects of LW on neurological function score, infarction volume of brain, thrombogenesis in vitro, platelet aggregation, tumor necrosis factor- α , pathomorphology of brain and the ultrastructure of neuron in rats. (3) Lysing effect of LW on blood clot was observed in vitro. (4) The global cerebral ischemia /reperfusion model was established to survey the influences of LW on the recovery time of righting reflex and electrical activity of brain , the water content and cerebral index , the activity of SOD , LDH, and NOS and the content of MDA, LD in brain tissue and ET in hippocampus . (5) The effects of LW on cerebral blood flow , cerebral vascular resistance , blood pressure, heart rate and electrocardiographic were detected in anaesthesia dog.Results (1) LW (52, 104, 208mg/kg) could prolond the gasping time of mice after quick decapitation and also prolond the survive time of anoxic mice. (2) Compared with model group, LW (52, 104, 208mg/kg) could decrease the neurological function score after focal cerebral ischemia at 8h and 22h reperfusion. LW (80, 160mg/kg) could decrease the infarction volume of brain in rats after focal cerebral ischemia reperfusion, relieve the brain tissue histopathologic injury and improve neuron ultrastructure . LW(80, 160mg/kg) could decrease the length , wet weight and dry weight of thromb and inhibit platelet aggregation . And also the expression of TNF- α could be inhibited by LW after focal cerebral ischemia-reperfusion in rats. (3) Lysing effect of LW(0.16,0.32mg/ml) on blood clot was observed in vitro. (4) The globe cerebral ischemia...