Comparison Of Vascular Endothelial Growth Factor Expression And Microvessel Density Between Pancreatic Carcinoma And Ampullary Carcinoma

Objective To analyse the expression of vascular endothelial growth factor (VEGF) and microvessel density (MVD) in pancreatic carcinoma and ampullary carcinoma for evaluating the effect and difference of angiogenesis in the development of these two tumors.Method Collection of 43 cases of pancreatic carcinoma sample (43 pericancerous normal pancreatic tissue as control) and 45 cases of ampullary carcinoma sample (45 pericancerous normal ampullary tissue as control), using immunohistochemistry (IHC) to detect the expression of VEGF and MVD.Result The positive rate of VEGF was 76.7% in pancreatic carcinoma and 53.5% in pericancerous normal pancreatic tissue, while MVD was 50. 01 ± 26. 33 in pancreatic carcinoma and 12. 66±5. 76 in pericancerous normal pancreatic tissue. These two parameters was significantly higher in tumor group than in pericancerous normal group (P<0.05 ) . In pancreatic carcinoma, VEGF and MVD in the group with lymph node metastasis was significantly higher than in the group without lymph node metastasis, as well as in the group with advanced TNM staging VS early staging (P<0.05) . VEGF expression in pancreatic carcinoma was found to be consistent with MVD very well (P<0.05) . In pancreatic carcinoma, survival time in the group with higher VEGF or MVD was significantly shorter than the group with lower VEGF or MVD (P<0.05) , MVD was the solo factor which can influent survival time independently (P<0.05) .The positive rate of VEGF was 73.3 % in ampullary carcinoma and 51.1% in pericancerous normal ampullary tissue, while MVD was 30. 91 ± 15. 75 in ampullary carcinoma and 14. 08±6. 54 in pericancerous normal ampullary tissue. These two parameters was significantly higher in tumor group than in pericancerous normalgroup (P<0.05) . In ampullary carcinoma, VEGF and MVD in the group with lymph node metastasis was significantly higher than in the group without lymph node metastasis, as well as in the group with advanced TNM staging VS early staging(P<0.05) . VEGF expression in ampullary carcinoma was found to be consistent with MVD very well (P<0.05) . In ampullary carcinoma, survival time in group with higher VEGF or MVD is significantly shorter than group with lower VEGF or MVD(P<0.05) .Our study shows that there was no significant difference in VEGF expression between pancreatic and ampullary carcinoma (P>0.05) , but MVD in pancreatic carcinoma tissue is significantly higher than in ampullary carcinoma tissue (P<0.05). Conclusion (l)VEGF is very important for angiogenesis, tumor growth and metastasis in pancreatic and ampullary carcinoma. (2)VEGF correlates consistently with MVD very well and both of the two parameters may be involved in tumor angiogenesis. (3)VEGF and MVD can be used as predictors for biological behavior and prognosis of these two tumors. (4)One of the reasons why the prognosis of pancreatic cancer is much poorer than that of ampullary cancer may be related to the hypervascularization in pancreatic carcinoma.