Study On Antimicrobial Susceptibility And Genotyping Of Plasmid Mediated Extended Spectrum Beta Lactamases And AmpC Beta Lactamases And Aminoglycosides Modifying Enzymes In Klebsiella Pneumoniae

Objective To investigate the antimicrobial susceptibility and genotyping of plasmid mediated extended spectrum beta lactamases (ESBLs) and AmpC beta lactamases (pAmpC) and aminoglycoside modifying enzymes (AMES) in Klebsiella Pneumoniae (Kpn) isolated from Huanhu hospital of Tianjin. Methods A total of 60 Kpn strains were tested to 21 drugs in vitro susceptibility by Microscan MIC method. The PCR and DNA sequencing methods were used to determine the genotypes of ESBLs, pAmpC and AMES. Results The 60 strains were multi-resistant drug. Three beta-lactamase genes and three AMES genes were found from 60 strains. There were TEM type broad-spectrum beta lactamases gene in 60 strains, CTX-M type ESBLs gene in 25 strains , DHA type pAmpC beta lactamases gene in 54 strains and AMES genes in 59 strains. CTX-M type ESBLs , DHA type pAmpC beta lactamases and AMES genes simultaneously occurred in 21 strains. Conclusions The resistance of Kpn isolated from HuanHu hospital in Tianjin was a serious issue and at least three kinds of beta lactamases and three AMES genes exsited.