Induction Differentiation Of Rosiglitazone In Human Gastric Carcinoma Transplanted Into Nude Mice

Objective To investigate the growth and induction differentiation effects of human gastric carcinoma which human gastric lower-differentiation mucinous carcinoma MGC-803 cells transplanted into back subcutaneous of nude mice by using PPARγ ligand rosiglitazone(ROS) and all trans retinoic acid(ATRA). And to preliminarily explore the mechanism of differentiation of ROS.Methods Firstly, cultivated the MGC-803 cells and collected them to be injected into back subcutaneous of nude mice(before operation weighing the mice), and observed the growth of the xenograft tumor, then screening the neoplasia mice. Secondly,The mice were divided into six groups: normal(A), model(B), ATRA 11mg/kg(C),rosiglitazone 25mg/kg(D1),rosiglitazone 50mg/kg(D2), rosiglitazone 100mg/kg(D3). There were five mice per group, when the xenograft tumor was about 100mm~3 beginning to use medicine.Both group A and group B ate and drank freely, others were poured the medicine into stomach. Measured the tumor volumes and weighed the mice every five days, evaluated ill effect of the medicine. Thirdly, after used medicine for forty days, sacrificed the mice and tumor volumes were measured under the dissection microscope and inhibition tumor rates were calculated; Observed morphology and angiogenesis of the xenograft tumor by HE stain; The cell cycle was detected by FCM;.The protein expression level of Mucin 5AC was detected by immunohistochemical.Results: 1. The weight of nude mice:before sacrificed the weight of nudemice: B were much lighter than other groups(p<0.05); And the weight were no difference between the A and other groups(p>0.05).2. After medication the effect to xenograft tumors: the difference of tumors...