Protective Effect And Its Mechanism Study Of Aminoguanidine And Vitamin C On Diabetic Rats' Blood Vessel

Objective: To study the dynamic change and inter relationship of the effects of Aminoguanidine and Vitamin C on microvessels (Kidney), extra cellular matrix IVα1 mRNA of large vessels (Aorta), lamininin B1 mRNA, fibronectin (FN) and heparin sulfate proteoglycan (HSPG) in the process of streptozotocin-induced diabetic rats in protein level, this paper explored the protective effects of Aminoguanidine and Vitamin C on blood vessel of diabetic rats from which new ways and methods for clinical medication of diabetes angiopathology were provided in the light of mechanism analysis.Methods: Type 1 diabetic mellitus rats, induced by intraperitoneal injection of STZ, were allocated to normal control group, model group, Vitamin C group, Aminoguanidine group, Vitamin C and Aminoguanidine group at random. All rats were treated with corresponding drugs for 16 weeks. Its general state, blood sugar, glycosylated hemoglobin, blood lipid, blood urea nitrogen, serum creatinine, creatinine clearance rate and 24-hour urinary albumin excretion rate were observed during and after the treatment; Serum collage IV and serum lamininin were detected by radio-innunity; Alteration of tissue morphology via H.E staining was observed after the kidney and aorta were taken out from the rats executed by narcosis; Fibronectin and heparin sulfate proteoglycan protein, collage IVα1 mRNA and laminininB1 mRNA were determined by immunohistochemistoche and hybridization analysis. Results: (1)Renal and aorta wall lesion occurred in the four model groups(2) Aminoguanidine and Vitamin C had no effects on blood gloucose, insulin and blood pressure, but they could improve the general state; Besides, they can decrease diabetic...