Study On The Level Of Serum S100-B Protein In Patients During CABG With Versus Without Cardiopulmonary Bypass

ObjectiveTo investigate the mutative law of the serum S100-B protein in patients undergone coronary artery bypass grafting(CABG) with cardiopulmonary bypass(CPB) and without CPB, compare the degree of cerebral injury in patients during CABG between the two different surgical manipulations, and probe into the factor working on the level of serum S100-B protein concentration.Methods30 patients were divided into two groups : general operation group of CABG with CPB (Group A) and minimally invasive operation group of that without CPB(Group B), and either group included 15 patients. The patients in Group A underwent the operation of CABG with CPB, and those in Group B did without CPB. 4ml blood of jugular vein was phlebotomized from every patient at the following 8 time points: pre-operation, post-anaesthesia, the time when the side-aortic forceps open, the end of operation, 2 hours after operation, 6 hours after operation, 12 hours after operation, and 24 hours after operation. The blood samples were centrifuged atthe speed of 3000rpm for 10 minutes and serum samples were saved in the special refrigerator(-70 ℃). The serum concentration of S100-B protein in the samples were measured with the measure of ELISA, and the times of Cardiopulmonary Bypass in Group A and the manipulative times on the heart in Group B were registered. All the data were analyzed using the software of SPSS10.0 for Windows.ResultsThe two groups had similar preoperative characteristics and there were no cases of deaths or prominent neurological complications in both groups. The level of serum S100-B protein increased markedly at the beginning of CPB, and the peak value was treble higher than that of the patients in Group B. The concentration of S100-B protein in serum increased only slightly during the CABG operation in the patients of Group B. The level of S100-B protein tended to be normal level in both groups 24 hours after operation. The level of S100-B protein during the operative process was not influenced by the anesthetic and the manipulation, but had notable relation with the time of cardiopulmonary bypass in Group A ( r=0.659, t=3.160, P =0.008) and the manipulative time on the heart in Group B ( r=0.584, t=2.190, P =0.022) .Conclusion1. The peek values of serum S100-B protein in patients of Group B is magnificently lower than that of Group B and the latter is treble times higher than the previous. It is can be concluded that The minimally invasive coronary artery bypass grafting without CPB can reduce the degree of cerebral damage and avoid functional disorder of the central nerve system because the physiological state in the operation is closer to the normal.2. The value of S100-B protein during the operative process was not influenced by the anesthetic and the manipulation, but had notable relation with the time of CPB inGroup A and the manipulative time on the heart in Group B. CPB and the manipulation on the heart may be the major factor to the cerebral damage during the operation.