Expression And Clinical Significance Of CyclinD₁ P16 Rb And Mart-1 In Congenital Cholesteatoma And Subsequent Cholesteatoma

Objective:To investigate the expression and distribution ofpl6> Rb and mart-1 in congenital , subsequent cholesteatoma and normalskin and disscuss their funtion in the souce and mobility ofcholesteatoma so as to find out the similarities and differences ofcongenital , subsequent cholesteatoma in the histopathobiology andprovide some acceptable theory indications for the etiology andtherapy of them.Methods: l19 cases of congenital cholesteatoma 30 cases of subsequentcholesteatoma from the first affiliated hospital of Fujian MedicalUniversity since 1998 as the experimental group, and 3 cases of normalauricle skin in the patients of chronic otitis media as the control groupwere drawn at random .The expression and distribution of cyclinD1, p16,Rb and mart-1 in congenital , subsequent cholesteatoma and normal skinwere studied by HE , special dye and immunohistochemistry.Results:1. result of HE and special dye1.1 result of HE More cells are found in subsequent cholesteatoma. Cholesterol crystal, both sequestrum and neogenesis bone , gland metaplasia, Polykaryocyte are found in subepidermis of subsequent cholesteatoma. While they are not found in congenital cholesteatoma.1.2 result of toluidine blue scatter mast cells are found in the subepidermis of subsequent cholesteatoma. While they are not found in congenital cholesteatoma.2. Result of immunohistochemistry. 2.1 mart-12. 1. 1 mart-1 are expression in both normal skin and cholesteatoma.2.1.2 mart-1 are expression in subsequent cholesteatoma, while they are not expression in part of congenital cholesteatoma. 2.2 cyclin D1 , p16 and Rb2.2. 1 cyclin D1 , p16 and Rb were expression in cholesteatoma, while not in normal skin.2. 2. 2 The positive expression rates of cyclin D1 p16 and Rb in subsequent were significantly higher than that in congenital cholesteatoma. The difference of positive rates was significant statistically, (P<0.05) . Conclusions:The difference was significant statistically in HE , special dye, the expression and distribution of cyclinD1, p16, Rb and mart-1 in congenital and subsequent cholesteatoma .We can draw conlusions that congenital and subsequent cholesteatoma may have some differences in histopathobiology and part of subsequent cholesteatoma may originate from the metaplasia of pavement epithelium. cyclinD1, p16, Rb and mart-1 may play a role in the etiology, progression and metastisis of cholesteatoma and they can be used as indexes to determine the malignant degree and differences in histopathobiology of congenital and subsequent cholesteatoma .