The Relationship Among IL-1 Gene Polymorphisms, Helicobacter Pylori And Gastric Cancer Development In Fujian Province

Objective:1. To study the distribution of interleukin-1 gene polymorphisms inpatients with gastric cancer and patients with duodenal ulcer in Fujian Province. 2. Tostudy the relationship among IL-1 gene polymorphism ,Hp infection and gastriccancer development .Method : Patients were divided into two groups,gastric cancer (GC) and duodenalulcer (DU) . Patients with GC were recruited by age, sex, gastric cancer family history,diagnosis and pathological diagnosis. Patients with DU were excluded NSAIDadministration .IL-1 gene polymorphism was analyzed by PCR-RFLP. Hp infectionwas diagnosed by rapid urease test with gastric mucosa ,¹³C expiration test and serumHp-IgG test by ELISA . Serum pepsinogen (PG)I ,II was tested by TRFIA .Result: 1. In GC patients , the frequency of CC,CT and TT of IL-1B-511 were18.1%,48.6% and 33.3%. Allele A1, A2 and A4 of IL-1RN were found and genotypeA1/A1, A1/A4, A1/A2 and A2/A2 occupied 88.9%,1.4%,9.0%,and 0.7%respectively: In DU patients , the frequency of CC, CT and TT of IL-1B-511 were19.6%,58.8% and 20.6%. Allele A1 and A2 of IL-1RN were found and genotypeA1/A1 and A1/A2 occupied 90.2% and 9.8% respectively.. The frequency ofIL-1B-511 TT genotype in GC was remarkably higher than in DU . 2. There was astatistical difference between genotype TT and non-TT of IL-1B-511 in GC, but nodifference among genotypes of IL-1RN. 3. Hp infection in GC was remarkably lowerthan in DU. 4. Serum PGI level and PGI/II in GC was remarkably lower than DU . 5.There were no significant differences among IL-1 genotypes in GC based on Hpinfection rate , pathology diagnosis, tumor site , family history and serum PGI,II leveland PGI/II rate. 6. There was also no significant difference between Hp(+) gastriccancers and Hp(-) ones regarding pathology diagnosis, tumor site ,serum PGI,II leveland PGI/II rate. 7. However, gastric cancer was found more in those with genotypeIL-1B-511 TT than with non-TT genotype only in patients with Hp infection.Conclusion : 1. CT of IL-1B-511 and A1/A1 of IL-1RN were the major genotypeboth in GC and DU in Fujian Province. 2. IL-1 gene polymorphisms, TT ofIL-1B-511,was associated with gastric cancer. 3. There was no difference in IL-1gene polymorphisms between proximal GC and distant GC and no difference betweenHp(+) and Hp(-) GC patients. No relation was found between IL-1 genepolymorphisms and the family history of gastric cancer, and also no relation betweenIL-1 gene polymorphisms and the pathological type of gastic cancer. 4. There were nodifferences in tumor sites, pathology types and serum PG level between Hp(+) GCpatients and Hp(-) ones. 5. Serum I and PGI/II rate in GC was remarkably lower thanin DU. 6. Hp infection may cooperate with IL-1B gene polymorphisms in thedevelopment of gastric cancer.