The Study On Rapid Detection Of Bacterial In Burn Sepsis By 16SrRNA Gene Polymerase Chain Reaction

Objective: A method for rapid detection of bacterial infection was Explored to diagnose burn sepsis early in clinic . Methods:(1) 41 blood samples of 39 cases with suspect burn sepsis were collected in the department of burns of the first affiliated hospital of JiangXi Medical College according to some criteria.Every blood sample was divided into two parts.One was sent for blood culture(BC),the other was detected by way of 16SrRNA gene Polymerase chain reaction(PCR) with universal primers of bacterial and PCR products were analysed by electrophoresis using agarose gel .The rapidity and sensitivity of two methods was Compared in detecting bacterials of patients with burn sepsis. Meantime positive and negative control group was established in PCR and each group contained 6 blood samples .(2)Analysis of the outcomes of BC and tests of drug sensitivity. Results: (1) 17 of 41 blood samples were positive by the way of 16SrRNA gene PCR and its PCR products were predicted 1.2kb bands in length and 24 samples were negative and with no band.Meantime the PCR products of 6 positive control samples were all predicted 1.2kb bands in length ,while the PCR products of 6 negative control samples had not any band.9 blood samples were positive With the method of BC and 32 samples were negative.Of all the 9 positive samples With BC, 8 samples were also positive by the way of 16SrRNA gene PCR and one was negative. The positive rate of the way of 16SrRNA gene PCR(41.16%) is nearly two times of the way of BC(21.95%),P<0.05. The spent time on the way of 16SrRNA gene PCR is shorter than that on the way of BC.Every group of the former is about 6 hours while a sample of the latter is 12-24hours. The speed of the way of 16SrRNA gene PCR in detecting bacterial is obivously faster than that with the way of BC.(2)8 of 9 positive samples with BC were the infections of single Pseudomonas aeruginosa and only one was the infection of single staphylococci haemolyticus. Pseudomonas aeruginosa resists the broadspectrum antibiotics of the first line universally which are used presently in clinic in our hospital from the test reports of drug sensitivity. Conclusions: (1)The method of 16SrRNA gene PCR in detecting bacterial has the advantage of rapidity and sensitivity. (2) The main bacterial which leads to burn sepsis is the Pseudomonas aeruginosa after using antibiotics in the department of burns of our hospital . Pseudomonas aeruginosa resists the broadspectrum antibiotics of the first line universally which are used presently in clinic in our hospital from the test reports of drug sensitivity.