Cellular And Humoral Immune Responses To Cervix Carcinoma In Mice Induced By Liposomal Crude Butanol Extraction(CBE)-based Vaccine

Since liposomes(phospholipids vesicles) were originally introduced by Bangham in 1965 as models of lipid bilayer membranes,they have been widely applied as models for studying effector phases of immune responses. Liposomes serve as a new type of immunological adjuvants,they were not only proposed as carriers of antigens, but known to be effective as immunoadjuvants for soluble protein antigens and even artificial simple peptides. Encapsulating antigen into liposome was an effective method to improve the immunogenicity of tumor antigen. In this experiment the crude butanol extraction of U14 (U14-CBE) was prepared by 2.5% 1-butanol, and liposomes made up of PC, PE and Chol were producted. The liposomal vaccine encapsulating U14-CBE was prepared by a method of combinating of the thin-film hydration, ultrasonication and extrusion through polycarbonate filters with a pore size of 220nm. The Km mice were immunized i.p. on day1,day7 and day14. A group of mice were killed for taking fresh spleens to be observed on day7,day14 and day 24 respectively. The spleens were weight wet weight,then observed to determine the T and B cell immune responses resulting from liposomal vaccine encapsulating U14-CBE inducing by using histology, histochemistry and immunohistochemistry methods. In this experiment the splenic weight of the mice immunized with the liposomal vaccine were increased significantly.The histological and histochemical observations on lymphoid tissue of immunized mice showed that an enlargement of the periarterial lymphatic sheath (PALS) and an increase in the number of immunoblasts, an increase in the number of large pyroninophilic lymphoid cells, the augmentation of lymphocytic germinal center's activity,and an increase in the number of plasmcoyte in the spleen. The enlargement of PALS, increase in the number of immunoblasts and large pyroninophilic lymphoid cells in the spleen indicated that liposomal vaccine could induce the significant cellular immune response.;however, the augmentation of lymphocytic germinal center 's activity,and ,an increase in the number of plasmcoyte responsed that liposomal vaccine could elicit the significantal humoral immune response. The present study indicated that liposome,when encapsulated tumor antigen, can improve the immunogenicity of this antigen. Liposomal antigen-based vaccine could elicit the significant cellular and humoral immune response in mice.