The Polymorphisms Of Angiotensin I-Converting-Enzyme Gene And Aldosterone Synthase (CYP11B2) Gene In Pre-eclampsia

Objective To observe the relationship between the genotype and allele frequency of angiotensin I -converting enzyme(ACE) gene and aldosterone synthase(CYP11B2)gene in mothers and fetals and pre-eclampsia. Methods We collected 43 pregnant women with preeclampsia and 44 healthy pregnant women as contrast and analysed the relationship between the polymorphisms of angiotensin I converting enzyme(ACE) gene and aldosterone synthase(CYP11B2) gene in pregnancy women and fetals and pre-eclampsia using the technique of polymerase chain reaction and restriction fragment length polymorphism. Moreover we inspected the activeness of ACE using ultraviolet spectrometer.Results 1.No significant differences in allele and genotype frequencies of ACE gene were found between patients with pre-eclampsia and the normal control subjects.Allele D frequencies were 37.2% and 29.5% respectively. No significant differences in allele and genotype frequencies of ACE gene were found between patients with early onset pre-eclampsia and patients withlate onset pre-eclampsia. Allele D frequencies were 32.4 % -. 40.4 % respectively.Also no significant differences in allele and genotype frequencies of ACE gene were found between fetals in the two groups. Allele D frequencies were 47.1 % > 32.5 % respectively.2.No significant differences in allele and genotype frequencies of CYP11B2 gene were found between patients with pre-eclampsia and the normal control subjects.Allele C frequencies were 37.2 % -> 30.7 % respectively. The frequency of the C allele in patients with early onset pre-eclampsia was significant higher than that in patients with late onset pre-eclampsia and allele C frequencies were 50.0% ^ 28.8%respectively, P< 0.05.The frequency of the C allele in fetals whose mothers with pre-eclampsia was significant higher than that in fetals of the normal control subjects and allele C frequencies were 35.7 % ^ 22.5 % P<0.05.3.No significant differences in serum ACE level were found between patients with pre-eclampsia and the normal control subjects.But serum ACE level was significant higher in patients with genotype DD than that in patients with genotype II in patients with pre-eclampsia, P<0.05. Conclusion No relations were found between ACE gene genotypes in mothers and fetals and pre-eclampsia in this study.But serum ACE level was significant higher in patients with genotype DD than that in patients with genotype II in patients with pre-eclampsia.There was relation between CYP11B2 gene allele mutation in mothers and early onset pre-eclampsia.There was relation between CYP11B2 gene allele mutation in fetals and pre-eclampsia too.