Differences Between The Expressions Of Matrix Metalloproteinase-2,-9 In Preeclampsia And Nomal Placental Tissues

Objective: To study the possible relationship between expressions of Matrix Metalloproteinase-2, -9 and the pathogenesis of preeclampsia in which trophoblast invasions is impaired. Methods: MMP-2,-9 expressions were detected by immunohistochemistry (SABC method) in 20 normal term placentae and 20 preeclampsia placentae respectively. In addition, mRNA of MMP-2,-9 were analysised by real time PCR in both groups. Results :The indensities of both MMP-2 and MMP-9 immunostaining in preeclampsia placentae were significantly declined compared to those of normal term placentae (P<0.05). By relatively quantitative assay, mRNA expressions of MMP-2 and MMP-9 were found significantly higher in 10 normal placentae than those in 13 preeclampsia placentae (P<0.05). In addition, mRNA expressions of MMP-2 were also elevated compared to MMP-9 in term normal placentae (P<0.05). Conclusion: MMP-2 and MMP-9 may play key roles in the invasion of trophoblast cell. The decreased expressions of MMP-2 and MMP-9 in preeclampsia placentae may lead to impaired invasion of trophoblast cell, thus attribute to abnormal placentation and pathogenesis of preeclampsia.