| Objective In the present study, mice received a intramuscular injection of recombinant BlyS plasmid . Effects of recombinant BlyS on disease in lupus BALB/c mice will be observed.Methods Thirty 10-week-old female BALB/c mice, special pathogenfree(SPF), were divided into P, P+BlyS groups and control groups . P groups received a single intraperitoneal injection of 0.5ml of pristane. P+BlyS groups received a intraperitoneal injection of 0.5ml of pristane and two times of intramuscular injection of 100 μ g of recombinant BlyS in three week intervals . 6 control mice received a single intraperitoneal injection of 0.5ml of 0.9%NS. Sera were obtained monthly to detect ANA and anti-dsDNA antibody. Urine samples were tested monthly for protein concentration by using Albustix reagent strips. 8 months after injection, all mice were bled to death. Livers were excised from mice to observe . SPSS was used to finishthe statistical analysis of the detective value .Results ANA appeared insera at 3 months ,and anti-dsDNA antibody was appeared at 4 months in P and P+BlyS groups mice after pristane-treated. some of the mice developed significant proteinuria. Focal necrosis and lymphocytic infiltration were observed in light microscopy of liver sections from P and P+BlyS groups. There was no statistical difference in the semi-quantity of proteinconcentration of urine (P>0.05), but the incidence of severe proteinuria was different.There was no statistical difference in titer of ANA between P and P+BlyS groups (P>0.05). The incidence of ANA was identical between two groups. There was no statistical difference in incidence and titer of anti-dsDNA antibody between P and P+BlyS groups (P>0.05). There was no statistical difference in pathological changes of liver (P>0.05) , but the incidence of focal necrosis and inflammation locus in P+BlyS groups is morethan that in P groups. Conclusion The abnormal expression of the recombinant BlyS has effect on disease in lupus BALB/c mice .
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