| It is an extremely complex process that cutaneous wound healing is. Several different kinds of repair cells, inflamation cell, cytokine take part in this process. There lie extraordinary complex networks between cytokines, between those repair cells, and between cytokines and repair cells. This process involves in cell migrations, adhesion, communication, proliferation, and diffentiation. Under the accurate adjustment of our body, it is an ordered process too. The latest studies shows that several stem cells, which are mainly epidermal stem cell(ESCs), may play a pivotal role in it. It is believed that ESCs is a fundamental cell in this procession of repairaton. Consequently, to interpret modulate mechanism of ESCs migration and differentiation are key to cutaneous wounds' "perfect" repairation.It had been found that neuropeptide, which is mainly secreted by the ending of sensory nerve, could accelerate repairation by means of moderate the function of immune cells, switch on inflammation of nerve derived, promote the proliferation of repair cells. Our prophase experiments also found that some neuropeptide, such as substance P, could promote ESCs to recruit around the wounds and accelerate wound healing. In order to further investigate the relationship of SP and ESCs, we design two level experiments —in vitro and in vivo. In vivo, firstly, We establish the nomal rat full-thickness wounds model, and employ integrin β1 keratin 19(K19), keratin 15(K15), Brdu as ESCs markers to conform the existence of ESCs in the granulation tissue of Group Capsaicin (rats were subcutaneously injected capsaicin pre-injury to damage sensory neuron and block the secretion of neuropeptides), Group Control, and Group SP (rats were injected SP in the wound postinjury). Secondly, as diabetic skin lacking of SP due to extensive pathological changes of sensory ending, we make a hypothesis:one of the main reason of unhealing wounds on diabetic patients could be insufficient SP which lead to fewer ESCs recruitment around wounds. To prove this hypothesis, we investigate the thickness of epidermis and thedistribution characteristics of ESCs of diabetic rats in different period and their relationship to unhealing wounds of diabetic skin. And then, the induction of SP towards ESCs' recruition and migration at the edge of wounds were studied on diabetic full-thickness wounds rats model. In vitro, the expression of SP special receptors(NK-1R, NK-2R) on cultivated ESCs were investigate on protein level and genetic level by immunocytochemistry and RT-PCR. Thereafter, we employed integrin |
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