Study On The Effect Of Bone Mesenchymal Stem Cells On Wound Healing In Diabetic Rat

Objective The model of diabetes rat was established in this study. BrdU-labelled bone mesenchymal stem cells were engrafted into the wound of the rats by means of injection. After designing control group, the differences of two groups of wound healing rate were compared by statistical analysis. The distribution and differentiation of bone mesenchymal stem cells were observed in order to evaluate the influence on wound healing in diabetes rat.Methods (1)Establishment of the diabetes rat model: the use of streptozocin(STZ) by intraperitoneal injection once; (2)Separation, culture and purification of bone mesenchymal stem cells: the use of whole bone marrow culture method; (3)Calculating of the rate of wound healing: application of a transparent membrane and statistical analysis to the differences; (4)Histopathological observation of the wound tissue was completed by Hematoxylin-eosin (HE) staining; (5)The distribution and differentiation of bone mesenchymal stem cells: the specimens around wound were immunohistochemically stained and cell differentiation was observed in serial section.Results (1)The successful construction of the wound model of diabetes rat; (2)Bone marrow mesenchymal stem cells (MSCs) were isolated, purified and cultured successfully; (3)According to statistical significance, bone marrow mesenchymal stem cells increased significantly the rate of wound healing in diabetic rats; (4)Compared with control group, the newborn epidermis of experimental group was thicker, cell layers and the layer were more obvious. Basal cells were arranged of single-layered columnar structure, combined tightly and integrated into organizational structure. Many skin appendages were observed in the dermis; (5)BrdU positive cells with brown nuclei aggregated in the epidermis, dermis and hypodermia. Some positive cells with brown cytoplasm appeared in the sebaceous glands and sebaceous duct cells and expressed keratin simultaneously in serial sections.Conclusion BrdU-labelled bone mesenchymal stem cells were injected into the wound of the rats. These cells have the potential for differentiating into epidermal cells and skin appendages under the microenvironment of diabetic wound. Bone mesenchymal stem cells can improve wound healing rate and promote diabetic wound healing.