Expression Of HSP70 In Intestinal Epithelium Of Scalded Rats During The Early Stage Of Burn And The Effect Of Rhubarb

Objectives: To observe the pathological changes and the expression of HSP70 in intestinal tissues after the rats were inflicted with scalded wound. To study the regularity of HSP70 expression in intestinal epithelium tissues and the effect of rhubarb on the expression of HSP70. To approach the protective mechanism of rhubarb on intestinal epithelium tissue injury. Methods: Prospective and randomized trial. The rats were divided into three groups: control group, burn group, rhubarb + burn group (termed as rhubarb group). The rats of control group were bathed in water with temperature of 37 centigrade for 15 seconds. However the rats of burn group were inflicted with three grade burn and 30% TBSA in the water of 95 centigrade for 15 seconds. After scalded the rats of rhubarb group were infused with rhubarb fluid through gastro tubes within five minutes. Henceforth those rats were infused with rhubarb twice a day at a dose of 150mg / kg. The rats of control group were killed 6h 12h 24h . 48h after scalded and the intestinal specimens were gathered. The specimens of burn group and rhubarb group were gathered receptively at the point of 6h 12h 24h48h after burn. All small intestinal tissue samples were promptly rinsed in cold saline solution and immediately fixed in 10% buffered formalin phosphate. Then the samples were embed in paraffin, sectioned transversely. Some were stained with hematoxylin-eosin for histological assessments of mucus damages and others were stained with immunoglobulin classes of rats that react with HSP70 for assessments of HSP70 expression. Results: Pathology by HE staining revealed that small intestinal epithelium tissues of burn group were damaged obviously after burn but the small intestinal tissues of control group were integrated and had no pathological changes. The damages of the rhubarb group were relieved obviously. The immunohistochemistrical assessments suggested that expression of HSP70 increased much more remarkably than that of control group at the point of 6h 12h 48h after burn (P<0. 01 ) . Furthermore, the intestinal tissues of rhubarb group had still higher levels of HSP70 expression than that of burn group at the point of 6h 12h 48h after burn (P<0. 01) . Conclusions: Rhubarb has therapeutic effect on burn rats, which can ameliorate the intestine injury after burn. Expression of HSP70 in intestinal tissues increases after burn and changes of the expression also show us. Rhubarb can enhance expression of HSP70 in intestinal tissues of the scalded rats. Overexpression of HSP70 induced by rhubarb maybe an important mechanism for rhubarb to protect intestinal mucus barrier.