| Objectives: The changes were observed of tumor necrosis factor(TNF-a), interleukin-6 (IL-6), myeloperoxidase(MPO), xanthine oxidase(MDA), malongialdehyde (XO) in liver tissue and their correlationship as well as the function changes in liver secretion and excretion during ischemia-reperfusion injury. The possible mechanisms of hyperbilirubinemia resulting from ischemia-reperfusion were Investigated. Methods: A model of liver ischemia-reperfusion injury was established. 48 healthy Wistar rats were randomly divided into 6 groups as following: the control group (the group of sham operation), simply ischemia 30min without reperfusion (â… group), reperfusion following ischemia 30 min (I/R group), 1 hr reperfusion following ischemia 30 min(I/Rlh group), 2 hrs reperfusion following ischemia 30 min(I/R2h group) and 4 hrs reperfusion following ischemia 30 min(I/R4h group). Each group involved 8 rats, including four females and four males. The radio-immunity method was applied to determine the contents of TNF-a and IL-6 in liver tissue and the chromatometry to measure contents of MPO, XO, MDA. The blood level was measured of total bilirubin(TB), conjugated bilirubin (CB) and unconjugated bilirubin (UCB) as well as total bile acid(TBA) from blood plasma. Live histopathologic changes were observed by the HE stain. Results: Ischemia-reperfusion resulted in significant liver injures. With the extension of reperfusion time, theHE stain of liver tissue showed the liver cell edema, vacuolar degeneration, inflammatory cell infiltration and even lamellar liver cell necrosis. In the content of TNF-a in the liver, statistical difference was in I/R, I/Rlh, I/R2hs and I/R4hs groups respectively compared with the control or I group(P<0.01), and also I/Rlh and I/R2hs with I/R (P< 0.05), I/R4hs with I/R (P<0.01), but no statistical difference in I/R2hs and I/R4hs with I/Rlh (P>0.05). The content of TNF-a in I/Rlh group was higher. In the content of IL-6 in the liver tissue, there was statistical difference between I/R and control group(P<0.05), and also I/R and I group, I/Rlh or I/R2hs and I/R (P<0.01), but no difference between I/R2hs or I/R4hs and I/Rlh group(P>0.05). The content of IL-6 in I/Rlh group was higher. In the content of MPO, statistical difference was between I/R and control group (/><0.01), and also I/Rlh and I/R(P <0.05), but no difference between I/R2hs or I/R4hs and I/Rlh group(P > 0.05). Compared I/R with control group, the content of XO significantly increased and the difference was statistically significant (P <0.01) as well as I/Rlh, I/R2hs and I/R4hs with I/R (/?<0.05), but no differences were between I/R2hs, I/R4hs respectively with I/Rlh (P> 0.05). Compared I/R with control group, the content of MDA increased and the difference was statistically significant (P<0.05) as well as I/Rlh with I/R (P<0.05), and I/R2hs, I/R4hs respectively with I/Rlh (P <0.05), but no difference was between I/R4hs and I/R2hs (P>0.05). Positive correlationship existed in TNF-a and MPO, TNF-a and XO, TNF-a and MDA, IL-6 and MPO, IL-6 and XO, IL-6 and MDA, MPO and XO, MPO and MDA, XO and MDA. In the contents of TB> CB^ UCB> TBA in blood serum, the differences were statistically significant in I/Rlh respectively compared with control, I and I/R group (P<0.01), and also I/R2hs with I/Rlh(P<0.01), I/R4hs with I/R2h(P<0.05). Positive correlationship was in MDA and TB, MDA and CB, MDA and UCB, MDA and TBA. Conclusions: The contents of TNF-a and IL-6were increased during the process of ischemia-reperfusion injury in the liver tissue, meanwhile the liver cells were impaired by neutrophilic granu- locyte infiltration and a great quantity of oxygen free radical produced by both XO activation and neutrophilic granulocyte infiltration. With the extension of reperfusion time, the dysfunctions took place in liver secretion and excretion, and the level of TB> CEK UCB and TBA significantly increased. It suggested that liver ischemia-reperfusion injury may be resulted in Hyperbilirubinemia or hepaticogenic Hyperbilirubinemia.
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