Study On Anti-tumor Activity Of Triptolide' Co-action With Traditional Medicines

With the co-action of Triptolide with two traditional anti-tumor medicines Amycin orHydroxycamptothecine on human heptocarcinoma SMMC-7721 cell, death-inducingeffect of triptolide'co-action with these two traditional anti-tumor medicines wasevaluated. The vitro culture examination indexes included observation of cellappearance, trypan blue staining, MTT staining and DNA laddering.SD rats are easy to obstain. It is easy and economic to feed SD rats, most important ofall, the rate of tumor's survival is very high, while the rate of tumor' fadeaway is verylow. SD rats are ideal model to act as transplanted liver cancers. In this essay,afterinoculation of SMMC-7721 to SD rats, the way of weighing the effect of triptolide onSD rats' death was through the examination of the mass of tumor by the verniercaliper, and software was available to value the reliability of the number.Caspase family belongs to cysteine aspartate-specific protease. Normally caspase-3exists in the cytoplasm with the activity-free zymogen. When cells neoresis, thezymogen is activated. We can measure the expression of caspase-3 with theexpression of caspase-3 to investigate the molecular mechanism of this coaction ofthese chemicals.Compared with the control group, the living cells were reduced significantly after theapplication of triptolide's cooperation with two traditional anti-tumor medicines, thetumor's volume were smaller and increased slowly while the control group's tumorvolumes increased significantly. At the same time, the expression of caspase-3 risedsharply.As a result, a conclusion could be drawn: Triptolide displayed a co-action effect whencooperated with the two traditional medicines, and triptolide had obvious lifeinhibition and apoptosis induction both in vivo and in vitro, which was associatedwith the expression of caspase-3.