Expression Of Inducible Nitric Oxidesynthase In Prostatictissues Of BPH Canine Models And Its Relation To Androgen And Benignprostatic Hyperplasia

Objective: To determine the relation among the expression of inducible nitric oxide synthase (iNOS) with in pathologrcal change of benign prostatic hyperplasia (BPH) and the induction of prostatic hyperplasia in the castrated male canine was observed by using androgens and estrogens.Methods: The young canine were castrated and treated with deep intramuscular injections of androstanediol (25 mg three times per weeks) and estradiol, (0.25mg three times per weeks). Total weekly dosage in all instances was 75 mg for androgens and 0.75 mg for estrogens. The expression of iNOS in prostate was assessed by immunohistochemistry.Results: At 6-mo intervals all animals were sacrificed and the restuls showed that spontaneous and experimentally induced canine prostatic hyperplasia were resembled in the prostatic weights , volumes, pathology and stromal component. iNOS was expressed only in the glandular epithelium of the prostatic tissues and the gradually decreased expression of which was found with the advance of injections time of androgen (P<0.05)Conclusion: The results to follow indicate that BPH can be produced by the chronic administration of androstanediol and estradiol and androgen is negatively related to the expression of iNOS and which may contribute to the pathogenesis of BPH.