| Aim To investigate the protective effect of fluvastatin and its influence on ICAM-1 mRNA expression and NOS activity in ischemia reperfusion myocardium in normocholesterolemic rabbit.Method 24 rabbits are divided into three groups randomly and myocardial ischemia reperfusion model in rabbit was made . Rabbits were subjected to 45 min of regional myocardial ischemia and 2h of reperfusion. 10mgkg~-1d~-1 fluvastatin were oral administered for one week. Dynamic index of blood flow was recorded and analyzed. Serum activity of CK,CKMB,LDH,LDH-1 were measured and the activity of iNOS and tNOS in ischemic myocardium were detected. Infarcted sizes in heart were determined by dual staining with Evans-blue and TTC. The expression of ICAM-1mRNA in ischemic myocardium were detected with semi-quantitative RT-PCR.Result In comparison with control group, pretreatment with fluvastatin decrease LVEDP at the whole obeservated duration, and spontaneously increase ±dp/dt_max. Serum activity of CK, CKMB, LDH-1 and the ratio of myocardial necrosis area in control group were significantly higher than those in sham group, but heavily reduced in fluvastatin group. Increase of activity ratio iNOS/tNOS due to ischemia reperfusion is reduced significantly in fluvastatin group compare to control group(0.25±0.10 VS 0.61 ±0.13, P<0.01) . Increase expression of ICAM-1mRNA due to ischemia reperfusion is reduced significantly in fluvastatin group compare to control group . Conclusion Pretreantment of fluvastatin may reduce inflammation reaction in reperfused myocardium. And this may contribute to its protective effect from experimental myocardial ischemia reperfusion.
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