Effects Of Topical Treatment With 5-aminosalicylic Acid And Sodium Butyrate On Expression Of Trefoil Factor 3, Interleukin-1 β And Nuclear Factor κB In Trinitrobenzene Sulphonic Acid-induced Colitis In Rats

Background and Aims Butyrate enema have been shown to be effective in treatment of ulcerative colitis, but the mechanism of the effects of butyrate is not totally known. The present study evaluates effects of topical treatment of sodium butyrate(NaB) and 5-aminosalicylic acid (5-ASA) on the expression of trefoil factor 3 (TFF3), interleukin-1β(IL-1β) and nuclear factor κB (NFκB) in trinitrobenzene sulphonic acid (TNBS) induced colitis in rats.Methods Distal colitis was induced in male Wistar rats by colonic administration of TNBS and colonically treated with NaB, 5-ASA, combination of NaB and 5-ASA, and normal saline for consecutive 14 days. Colonic damage score, tissue myeloperoxidase (MPO) activity, TFF3 mRNA expression, serum IL-1β production and tissue NFκB expression were determined, respectively.Results Treatment of NaB, 5-ASA and the combination improved diarrhea, colonic damage score and MPO activities, enhanced TFF3 mRNA expression and decreased serum IL-1β production and tissue NFκB expression. The combination therapy of NaB and 5-ASA had better effects than any other single treatment.Conclusions The combination of topical treatment of NaB and 5-ASA was effective for relieving and repairing of colonic inflammation and the effects were related to stimulation of TFF3 mRNA expression and down-regulation of IL-1βproduction and NFκB expression.