| BackgroundRenal tumor is one of the most common tumors of human. According to the histological classification of WHO(2004), the epithelial tumors of kidney is including 10 kinds of malignant and 2 kinds of benign tumors. The clear cell renal cell carcinoma is about 70% among all the renal cancer in the corresponding period. Chromophobe renal cell carcinoma is a kind of relatively rare renal cancer, and it is about 4%-5% among all the renal cancer in the corresponding period. Because of its unique morphologic characteristic and the better prognosis, chromophobe renal cell carcinoma is isolated as a new type by WHO in 2004. Oncocytoma is a kind of benign epithelial tumor and occupy about 5% in all renal tubular tumors. Owing to limitation of understanding for renal tumors, many pathologists confuse these three kinds of tumors and give the wrong diagnoses.The genesis and progression of the cancer of kidney relate to many kinds of factors. Receptor tyrosine kinases(RTKs) RON(recepteur d'origine nantais) is the receptor of MSP(macrophage stimulating protein). RON participates many biological process in vivo such as the activation of macrophage, cell inducing immune response, cellular growth, movement and survival. Recent studies show that RON has some unique functions as cell proliferation, motility, and invasive growth and prevents theapoptosis of epithelial cells by overexpression, mutation or splicing. It is suggested that RON might involved in oncogenesis and progression of certain epithelium tumor in vivo. Therefore, there is important academic and practical significance for study on the function of pathogenesis. AimStudy on the histopathology classification and morphologic characteristics of the renal epithelial tumors. Explore the relationship of the expression of RON and infiltration, metastasis, the grade of differentiation of the renal tumors. Methods:(1) 71 cases were undertaken by histological classification, Fuhrman's nuclear grade according to WHO schemes of RCC from "Tumors of the Urinary System and Male Genital Organs" published in 2004.(2)Hale's colloidal iron staining for chromophobe renal tumors.(3) immunohistochemical staining for detecting expression of RON.(4) detect the expression of Ron gene in 32 cases of clear cell renal carcinomas by RT-PCR, and p-actin as the internal control.Results:(1) There are 48 cases of clear cell renal cell carcinomas, 20 cases of chromophobe renal cell carcinomas and 3 cases of oncocytomas among 71 cases renal tumors.(2) Results of Hale's colloidal iron staining: positive in all the chromophobe renal cell carcinomas.(3) Results of immunohistochemical staining: RON express in most celluar plasm and membrane of the tumor cell. In clear cell renal carcinomas, staining intensity is much higher in the cases of 3 to 4 grade of fuhrman than those of 1 or 2 grade, p<0.05. It is highest in the cases of chromophobe renal cell carcinomas, less in clear cell renal carcinomas the, and least in oncocytoma, but there were no significant differences. The expression of RON in the cases that tumor's diameter are over 7cmis higher than those are less 7cm. but it is also no significant different. There is a trend of higher expression in those cases of invading the renal pelvis.(4) Results of RT-PCR: the mRNA of ï¿¡ -actin were detected in the 28 specimens;and mRNA of RON were also detected in 22 among the 28 specimens Conclusions:1. According to the characteristic of morphology under microscope with HE staining, most of clear cell renal cell carcinoma, chromophobe cell carcinoma and oncocytoma can be differentiated correctly. Hale's colloidal iron staining is meaningful when it can not be made the diagnosis by regular examination.2. RON expresses in clear cell renal cell carcinomas, chromophobe cell carcinomas and oncocytomas. The expression of RON in renal tumors might relate to the grade of nucleus. It is higher of the grade of nucleus, the stronger of the expression. In the cases of invading renal pelvis, there is also a trend of stronger expression of RON.
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