Preliminary Study Of Proton Magnetic Spectroscopy In Hepatic Malignant Tumor And Heart Ex Vivo

Part 1:Title: Preliminary Study of Hepatic Malignant Tumors In Vivo by Proton Magnetic Resnance Spectroscopy at 3.0 TText: Objective To preliminarily study the characteristics of proton MR spectroscopy (¹H-MRS) of hepatic malignant tumors at 3.0T in vivo.Methods The ¹H-MRS findings of 14 cases of hepatic malignant tumors proved by pathology were analyzed retrospectively. In each case both of the lesion and correspondingly normal region were scanned respectively in the same examination before the operation. The concentration of choline and TG in all the regions of interests were calculated and compared respectively.Results 10 cases were proved to be HCC, 7 of them showed the concentration of choline substantially higher than those obtained from the correspondingly normal regions, while another 7 cases of HCC showed the concentration of TG higher than the correspondingly normal regions. 4 cases were proved to be other malignant tumors, 3 cases of them showed lower concentrations of choline and TG than the correspondingly normal region.Conclusion ¹H-MRS is technically feasible for the evaluation of HCC in vivo. The hepatic tumor showed the elevation of choline suggests the diagnosis of HCC.Part 2:Title: Assessment of Myocardial Fatty Degeneration Ex Vivo by Proton Magnetic Resonance SpectroscopyText: Objective To evaluate the feasibility and accuracy of ¹H-MRS on the assessment of myocardial fatty degeneration ex vivo.Materials and Methods 10 fresh hearts specimens within 72 hours after death were examined on 1.5T GE MR scanner, with sequences of ¹H-MRS was scanned in LAD, LCX and RCA distribution areas as ROIs (3 voxels) in mid-cavity of left cardiac ventricle respectively. The peak values of H₂O and TG were calculated with the software SAGE (GE healthcare), and calculated the percentage of TG: TG/(H₂O+TG) X 100%. The specimens were fixed with 10% of formalin solution for a week and then the pieces of myocardium that corresponding to the voxels were made into chips. The cadiocytes with fatty degeneration were observed and counted under light microscope (magnification ×40) and the corelation of the MRS assessments of TG% and counting of cadiocytes with fatty degeneration in the voxels of three distribution areas were analyzed respectively.Results Each spectrum was good for analyzing and the spectral peaks were identified with the following chemical shifts: water at 4.7 ppm, myoinositol at 3.5ppm, choline at 3.2 ppm, creatine at 3.0 ppm, and TG at 0.9-1.4 ppm. Neither the percentage of TG nor the cell countings' differences in distribution areas was statistic significant (F=0.142, P=0.868, and F=2.504, P=0.101). There was a positive correlation between the percentage of TG and cell counting (Spearman: r =0.557 P =0.001, Pearson: r =0.453, P=0.012).Conclusion Assessment of myocardial intracellular TG using ¹H-MRS ex vivo is feasible and there was a positive correlation between the MRS assesment of TG% and cell counting.