| objective: Angiogenesis plays an important part in the occurrenc and growth of malignant tumors. Vascular endothelial growth factor (VEGF) and basic fibroblast growth factor (bFGF) are the most powerful and specific factors promoting angiogenesis. This article was to evaluate the serum concentrations of VEGF(sVEGF) and bFGF (sbFGF) in patients with myelodysplastic syndrome(MDS) and their relationships with clinic factors, study their role in the diagnosis, progression, and prognosis of MDS, and provide theoretic evidence of clinic anti-angiogenesis therapy.Method: Collect 52 serum samples of 43 MDS patients and detect the concentrations of VEGF and bFGF with enzyme-linked immunoabsorbent assay (ELISA). Compare the serum levels of VEGF and bFGF with that in normal control and acute myeloid leukemia(AML) .The differences of sVEGF and sbFGF levels in the subtypes of MDS patients, the relationship between sVEGF level and sbFGF level, their relationship with gender, age, peripheral blood routine and the proportion of blast cells in bone marrow were also analysed.Results: The sVEGF level in 43 MDS patients is 77.11 ±21.37pg/ml, significantly higher than that in normal control group(77.11±21.37pg/ml)( P<0.01),lower than that in AML group(377.49±146.31pg/ml)(P<0.01).In the subtypes, the sVEGF level in RAEB group(269.62±118.71pg/ml) and RAEBT group(345.59±101.98pg/ml) are much higher than that in RA group(75.62±20.53pg/ml) and RAS group(75.78 ± 19.73pg/ml).The sbFGF level in 43 patients with MDS is 21.33 ±10.38pg/ml, significantly higher than that in normal control group(9.40±1.59pg/mlXP<0.01),has no significant difference with AML group(26.20±9.13pg/ml)(P>0.05).In the subtypes, the sbFGF level in RAEB group(27.17±10.70pg/ml) and RAEBT group(28.50±10.77pg/ml) are much higher than that in RA group(15.34±6.55pg/ml) and RAS group(15.27±3.64pg/ml).The sVEGF and sbFGF levels are related to peripheral blood routine and the proportion of blast cells in bone marrow. There is positive correlation between sVEGF level and sbFGF level in MDS patients. Conclusion: The sVEGF and sbFGF levels in MDS patients are significantly increased.In the subtypes, the RAEB and RAEBT patients have higher sVEGF and sbFGF levels than RA and RAS patients. The sVEGF and sbFGF levels are related to peripheral blood routine and the proportion of blast cells in bone marrow. To detect the s VEGF and sbFGF concentrations in MDS patients is conducive to the diagnosis, classification, assessing prognosis and directing therapy.Postgraduate student:Meiqing zhao (hematology) Directed by Prof. Hongguo Zhao, Fanjun Meng... |
