The Study On Antiviral Action Of Polysaccharides From Stichopus Japonicus

Objective: To explore antiviral action of polysaccharides from Stichopus Japonicus and analysis its' antiviral mechanisms.Methods: The polysaccharides was extracted with the alkali and enzyme hydrolyze assays, and identify its' character. Herpes simplex virus type I standard strain (HSV-1, SM44 strain) and Sendai virus (SV) were used to investigate the antiviral activities of polysaccharides in vitro and in vivo. Then various concentrations of polysaccharides were applied to different steps of virus replication cycle. Cytopathic effect (CPE), methyl thiazolyl tetrazolium (MTT) method and real time quantitative PCR were used as evaluation criterion. In vivo, the stomachs of KM mice those infected by HSV-1 virus or Sendai virus were filled with polysaccharides.Result: â‘  The polysaccharides sample is pure, and the ingredient of the Stichopus Japonicus acidic mucopolysaccharide has galactoamine, glucumonic acid, fucose and sulfate. â‘¡ The mechanisms of polysaccharides against HSV-1 and SV may be explained by the inhibition on virus adsorption and virus replication. â‘¢ We can not observe antiviral action of polysaccharides in the polysaccharides effecting the cells directly, virus penetration and polysaccharides pre-treated cells. â‘£ The results in vivo also indicate that polysaccharides can promote to product hemagglutination inhibition antibody, and degrade mortality of KM mice infected by HSV-1 virus and Sendai virus. Higher dose of the polysaccharides has better antiviral action.Conclusion: These results not only indicate that the Stichopus Japonicus acidic mucopolysaccharide exerts antiviral action in vitro, but also elevate immunological function of the KM mice, and protect the KM mice.