Effect Of Angelic-extract On Proliferation, Apoptosis, Collagen Synthesis Of Human Umbilical Vein Endothelial Cells And Fibroblasts

Objective: To investigate the effects of Angelicanaphtha and Angelic decoction on proliferation, cells cycle, apoptosis, and collagen synthesis of human umbilical vein endothelial cells(HUVEC) and fibroblasts. Methods : HUVEC and fibroblasts was cultured in DMEM medium.The proliferation was measured by MTT method. The cell cycle and apoptosis were analyzed by Flow cytometry and collagen synthesis was determined by radioimmunoassay. Results:1. Angelicanaphtha had dualistic effects on the proliferation of the HUVEC. The proliferation was accelerated by low concentration (<4mg/l) of Angelicanaphtha and inhibited by high concentration of Angelicanaphtha (≥16mg/l)(P<0.05). G₀/G₁ phase cells were decreased and S phase cells were increased and the apoptosis was inhibited by Angelicanaphtha of lmg/l and 4mg/l significantly (P<0.05). However, Angelicanaphtha of 16 mg/1 increased in G₀/G₁ phase cells and decreased in S phase cells (P<0.05) and induced the apoptosis (P<0.05). Thecollagen synthesis of HUVEC was inhibited by Angelicanaphtha in concentration-dependent manner (P<0.05 or P<0.01).2. With the concentration increasing, the inhibitory action of Angelicanaphtha on the fibroblasts proliferation became even more obvious. The inhibitory roles of Angelicanaphtha of 16mg/l were the best and suppression rate was the biggest at the 3rd day (reached 43%). Angelicanaphtha of 4mg/l and 16mg/l made G0/Gi phase cells, G2/ M phase cells decrease and S phase cells remarkable increase (PO.05). Furthermore, apoptosis of the fibroblasts was increased with increase of Angelicanaphtha concentration (P<0.05). The collagen synthesis of fibroblast was inhibited by Angelicanaphtha in concentration-dependent manner (P<0.05).3. The HUVEC proliferation was accelerated by Angelic decoction in concentration-dependent manner (P<0.05). S phase cells and G2/M phase cells were increased and G0/G1 phase cells and apoptosis was gradually reduced by Angelic decoction (P<0.05). The collagen synthesis of HUVEC was also inhibited by Angelic decoction in concentration-dependent manner (P<0.05).4. The fibroblasts proliferation was inhibited by Angelic decoction in concentration-dependent manner. 400mg/l and 1600mg/l Angelic decoction made G0/Gi phase cells, G2/M phase cells reduce and S phase cells remarkable increase (P<0.05). The apoptosis also increased with theconcentration of Angelic decoction up (P<0.05). However, the collagen synthesis of fibroblast was inhibited by Angelic decoction in concentration-dependent manner (P<0.05). Conclusions:1. The proliferation effects of Angelicanaphtha on HUVEC had dualistic regulatory roles: increase by low-concentration and inhibition by higher-concentration. Collagen synthesis of HUVEC was inhibited by Angelicanaphtha in concentration-dependent manner.2. The proliferation and collagen synthesis of fibroblasts was inhibited and apoptosis was accelerated by Angelicanaphtha in concentration-dependent manner.3. The HUVEC proliferation was accelerated and the apoptosis was induced and the collagen synthesis was reduced by Angelic decoction.4. The fibroblasts and the collagen synthesis of proliferation were inhibited and the apoptosis was induced by Angelic decoction in concentration-dependent manner.