Effects Of OCT4 And BFGF On Proliferation And Migration Of Human Umbilical Vein Endothelial Cells And Molecular Mechanism Involved

ObjectiveIn this paper, we study the effect of Oct4 and b FGF on human umbilical vein endothelial cell,to elucidate the basic molecular mechanisms of regulation of vascular endothelial cell proliferation and migration, providing new ideas for arsenic disease vascular injury repair MethodsTet O-FUW-OCT4 system induced by DOX was used to make the overexpression of OCT4 in HUVECs, and b FGF was added to culture in vitro. Cell proliferation and cell migration was detected by CCK8 assay and scratch test method. gene chip analysis were used to detect the differentially expressed genes in the whole genome wide range, and the resμlts were verified by real-time quantitative PCR. Resμlts1. Cultured continuously for days, CCK8 assay detected cell proliferation, add DOX cultured HUVECs in the experimental group at the fourth day, OD began was significantly higher than that of the control group. And this kind of phenomenon was not occurred in the HUVECs experimental group added with b FGF cμlture.2. Scratch migration test, in the b FGF cμltured HUVECs 24 h experimental group, the cell migration distance was significantly higher than the control group. And there was no significant difference in the HUVECs experimental group added with DOX.3. Gene chip analysis of the group differences in gene expression, biological processes were classified according to gene and HUVECs differences in the role of Oct4 expression genes screened associated with proliferation of 323 differentially expressed genes, which were up-regulated with 172, the cut is 151.4. Gene chip analysis of the group differences in gene expression, and according to the biological process classification of gene in the mechanism of b FGF HUVECs difference expression genes were screened with migration 36 differentially expressed genes, which raised a 19, the cut is 17.5. RT-QPCR results verify that OCT4 after overexpression, EGR1, JUN, FOS of Pathway Mitogeneic in HUVECs, and WISP1 of Pathway Wnt is over expression.6 RT-QPCR results show that HUVECs, MMP3, KRT13 and MMP1 expression levels in b FGF culture are relatively higher, while the CHD22 expression level is relatively lower. Conclusion1. OCT4 participate in the regulation of the proliferation mechanism of HUVECs, and play a significant role in promoting. On the contrary, it may not have a significant effect on the migration of HUVECs.2. b FGF not be involved in the regulation of the proliferation of HUVECs, however, in the migration behavior of HUVECs, may play a more significant role in promoting.3. EGR1 and WISP1, FOS and JUN may be involved in the regulation of HUVECs proliferation as a downstream target gene of OCT4.4. MMP1, MMP3 and CDH22, KRT13 may serve as the downstream regulation network of b FGF promoting HUVECs’ migration.