Immobilization Of Fibronectin And Its Influence To Biological Behavior Of Endothelial Cells Onto Titanium-Oxide Films

In this paper, the titanium oxide films with Rutile structure were synthesized by unbalanced magnetron sputtering and then modified by one surface biomodification method. The titanium oxide films were activated by HCL and H₂O₂ to produce hydroxyl group, then coated with poly-L-lysine to promote the adsorption of biomacromolecule and immobilized with fibronectin. The surface characteristics of samples were investigated using Fourier transfer infrared spectrum (FTIR), X-ray photoelectron spectroscopy (XPS), Atom force microscope (AFM) and Scanning electron microscope (SEM). The wettability of Ti-0 films were studied by a contact angle method. The biological behavior of cultured human umbilical vein endothelial cells (HUVECs) onto different samples was evaluated by the in vitro HUVECs cultured experiment. This work focus on the surface modification of immobilization of fibronectin and its influence to the biological behavior of cultured HUVECs onto titanium-oxide films.The results of FTIR indicated that the titanium oxide film can produce hydroxyl group activated by HCL and H₂O₂. The peak of O element corresponding to hydroxyl group increased in results of XPS, the contact angle was lower.The results of FTIR and XPS indicated that the hydroxyl group can react with carboxyl of poly-L-lysine to make it bond chemically with film surface. The results of SEM and AFM indicated that the surface morphology changed with the appearance of little grains in size of 100nm and contact angle of the surface increased.After immobilized with fibronectin, the surface of titanium oxide film can adsorb fironectin which showed in the results of FTIR and XPS. The contactangle became larger.The in vitro HUVECs cultured investigation justified that the endothelial cells have affinity with Ti-0 film, fibronectin can promote the adhesion and growth of endothelial cells, the results of HUVECs cultured experiment of Ti-0 film which modified by poly-L-lysine and immobilized with fibronetcirr is better than that of Ti-0 film immobilized directly with fibronctin.All these results indicated that fibronetin can promote the adhesion and growth of endothelial cells, the method of coating with PLL and immobilizing with fibronectin can promote the adhesion and growth of endothelial cells. This method provided a surface biomodification way for improving the biocompatibility of Ti-0 film.