| During the past several years, the study of biological effect after low doseradiation(LDR) make extraordinary program. Compare to large dose radiation,low dose radiation (LDR) have the different biological effect that were"hormesis" and "adaptive response". They are of considerable practical valuesin the clinical application. At present individual proteins were paid attention to in the study of thethe mechanism of LDR, while there are many events in this biological effect,So the high-flux and all round method must be used to study the mechanismof LDR. With the proteomic technology exploitation and improvement, wecan explore the rules of the protein in the whole level. At present there are fewstudies in the mechanism of biological effect on blood system induced byLDR. So,in this dissertation the mechanism of LDR was studied by proteomictechnology to analysis the differentially-expressed proteins between the groupafter LDR and the control group in order to find the key proteins of thehormesis effect and adaptive response on blood system induced by LDR,Which directly shows the change of the group-accommodation in the cells.That may provide a new method to study the mechanism of LDR.According to the result of our preceding study about LDR, we used75mGy to irradiate mice, then at 24, 48and 72 hours later killed the mice toresearch the differentially-expressed proteins of serum and bone marrowhematopoietic stem/progenito cells, after LDR. Two-dimensionalelectrophoresis (2-DE) was used to screen protein patterns of normal and themice subjected to LDR in different time for qualitative and quantitativedifferences in protein expression. And the differentially-expressed proteinsbetween the two groups were identified by matrix assisted laserdesorption/ionization-time of flight-mass spectrometry (MALDI-TOF-MS),then through the database search to find the key proteins of the hormesis andadaptive response induced by LDR. These methods were used study themechanism of biological effect on blood system induced by LDR in wholelevel.Among the differentially-expressed proteins of mice serum between thegroup subjected to LDR and the control group, it was found that after LDR4proteins appeared, 13 proteins were taken up-regulated, 6 proteins weretaken low-regulated, 3 proteins disappeared. Among thedifferentially-expressed proteins of the mice bone marrow hematopoieticstem/progenito cells, it was found that after LDR 26 proteins were takenup-regulated, 4 proteins were taken low-regulated and 4 proteins disappeared.In different time the quantity of some proteins was different. Somecharacteristics of the proteins expression can be found. Thedifferentially-expression of proteins were most obvious in 48 hourspost-irradiation group. It shows that biological effect on blood system inducedby LDR is related to time.Identified by MALDI-TOF-MS to the differentially-expressed proteinsbetween the two groups in the mice serum, We found estrogen receptor 2,vitamin D-binding protein ,Apoa1 protein and so on 6 kinds of proteins. In thebone marrow haemopoietic stem/progenito cell, the 22 kinds of proteins wereidentified, including Laminin receptor 1, purine nucleoside phosphorylase,Nonselenium glutathione peroxidase, carbonic anhydrase 2, Chlorideintracellular channel 1, heterogeneous nuclear ribonucleoprotein A2/B1,Protein disulfide isomerase associated 3, ARP1 actin-related protein 1homolog A, Phosphogluconate dehydrogenase, fibrillin-1, Tu translation,mitochondrial elongation factor, mitochondrial precursor, Serpinb1a proteinand so on. They play a part in the mechanism of biological effect on bloodsystem induced by LDR though influence on the metabolic reaction of cellcycle, signal transmission, cystoskeleto, metabolism, alarm reaction and so on.The study shows that there are 26 kinds of proteins in the mice serum and34 kinds of proteins in the mice bone marrow hematopoietic stem/progenitocells, to change in expression. Now, we have identified parts of these proteins,we can infer that these proteins play some important roles in the mechanismof biological effect on blood system induced by LDR. As for the proteins thatwe cannot identify temporary, we have gotten their The isoelectric point andmolecular weight, we will continue to identify them through miss spectrum,amino acid sequence analysis, bioinformatics search and so on. Which rolesthese proteins place in the mechanism of biological effect by LDR? Weshould research its structure and function. It is the work that we is in progressand is going to do. In this dissertation, we used proteomic technology to studyThe mechanism of biological effect on blood system induced by LDR inwhole level. That may provide a new and effective method to explanation forthe mechanism of the low dose radiation, which provides the foundation ofexperiment and theory for the clinical application of LDR. |
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