The Protective Effect Of Electroacupuncture At "Zusanli" Points On Hemorrhagic Shock Through Cholinergic Anti-inflammatory Pathway

Objective To investigate the effect and meschanism of electroacupuncture at "Zusanli" points on hemorrhagic shock in rats.Methods 50 SD rats were randomly divided into ten groups:' sham group, hemorrhagic shock group, non-acupoints group, "Zusanli" points group, bilateral cervical vagotomy group, vagotomy and electroacupuncture group, muscarinic antagonist group, nicotinic antagonist group, α-BGT group and THA group. Hemorrhagic shock was induced by intermittent withdrawing of blood until mean arterial pressure(MAP) stabilized within the range of 25 to 30 mmHg. Electroacupuncture at "Zusanli" points with constant voltage(2~4V,3Hz) for 30 minutes immediately after the shock models were made. Before electroacupuncture a blood pressure transducer was implanted in the common carotid artery for continuous registration of MAP. Blood samples and liver samples were collected from animals of all groups. Serum levels of tumor necrosis factor-α(TNF-α), interleukin-10(IL-10) and liver nuclear factor kappa-B(NF-κB) were determined. Another 40 rats were randomly divided into four groups. Record survival time and rate.Results In hemorrhagic shock group MAP was markedly lowered, survival time and rate were shorten, and the content of serum TNF-α, IL-10 and liver NF-κB markedly increased after the shock models were made. The above indexes in non-acupoints group were not different from those of hemorrhagic shock group(P>0.05). But electroacupuncture at "Zusanli" points significantly reverted the marked hypotension, increase the survival time and prompt the survival rate, reduced serum TNF-α and blunted liver NF-κB activation(P <0.01). And muscarinic antagonist group still had such effects(P<0.01). But vagotomy and electroacupuncture group, nicotinic antagonist group and a-BGT group had no such effects(all P>0.05). And compared with the level of IL-10 in hemorrhagic shock group, it did not show significant differences in other groups(all P>0.05).Conclusions The results suggest that electroacupuncture at "Zusanli" points might produce a potential protective effect on hemorrhagic shock in rats through the cholinergic anti-inflammatory pathway.