| BACKGROUND AND PURPOSE:The lung cancer disease incidence rate is advancing year by year, to its diagnosis treatment and prognosis judgment still not entirely as desired. The lung cancer occurrence and development are more than one kind of factors, the multi- steps process, in which participates in the adjustment factor and the link are very much. Some literature reported the HSP70 expression elevated with droping the Fas expression in the non-small cell lung cancer (NSCLC) in the occurrence and development could be the certain function.The heat shock protein(HSP) is also called as Stress protein (SP), at present discovered HSP has 10 extra kinds, the content of HSP70 is the most HSP and HSP70 also is most important HSP. Known HSP70 is one kind of non- specificity cell protection protein, has the maintenance cell protein from to be steady, enhances the cell correspondence to stir up the original tolerance, has function which the suppression cell apoptosis . Had the literature to report that, the heat shock protein 70 expressions advanced in some malignant tumors, and thought HSP70 the expression level might take the tumor differentiation degree, the clinical prognosis, antibiotic and so on judgment target.Fas called APO-1 or the CD95 member, is one kind of membrane acceptor, belongs to the tumor necrosis factor acceptor family, has induces the cell apoptosis ,maintenance organism own is stable and in the maintenance the environment balance and so on the vital role, recent years studied indicated Fas expression level in the many kinds of tumors cell was declined or flew, in order to evaded the host immune cell induction apoptosis .Studies Fas in the non- small cell lung cancer expression research was quite much, but studies HSP70 was few in the non- small cell lung cancer expression. Examines HSP70 and in jointly the Fas literature in the non-small cell lung cancer was rare, has not seen has the relevant analysis. Moreover the immunohistochemical techniques is easy in the basic unit hospital to develop, is advantageous for the promotion.Therefore we use the immunohistochemstry, study HSP70 and Fas in the non-small cell lung cancer tissues expression and with the clinical pathology characteristic relations, analyze HSP70 and Fas in the tumor expression relevance.The generalized analysis to research result of HSP70 and Fas could be used as a useful marker of a supplementary diagnostic and prognosis for patients with NSCLC. Examination of expression of two proteins may be helpful to judge biological behavior and prognosis of non-small cell lung carcinoma.Methods and Materials: 46 examples non-small cell lung cancer is the patient which in April, 2005 in our hospital, the masculine 34 examples, the feminine 12 examples, aging from 43 to 77 with a mean age of 60.61 years old. All specimens pass through the pathology confirmation. In front of technique not after radiotherapy either chemotherapy or high temperature treatment. At the same time chooses 10 examples same time the benign pathological change surgery patient to be far away the normal lung tissue which the pathological change organizes as the comparison research. The specimen is fixed after 10% neutral formalin, conventional paraffin wax embedding, continuously 4 u m thickness slice. The immunity group dyeing uses SP dyeing, the concrete operation according to the SP reagent box explained carries on. Under the light mirror observes HSP70 and Fas in the cancer tissues and normal tissue's expression situation. Software carries on data statistics processing using SPSS 12.0 for the windows.Results:1. Positive rate of Hsp70 and Fas expression in human non-small cell lung carcinoma was 80.43%(37/46),65.22%(30/46) respectively. Positive rate of Hsp70 and Fas expression in normal lung tissues was 0.00% ,100% respectively.2. The expression of Hsp70 was closely related to lymphatic metastasis but not related to tumor's cell types,clinlcal staging and pathological grade. The expression of Fas was significantly related to tumor's clinical staging ,pathological grade and lymphatic metastasis, but not related to tumor's cell types.3. The level of hsp70 expression was negatively related to that of Fas (p<0.05). Conclusion: The overexpression of Hsp70 and the lowexpression of Fas correlate with the development and metastasis of non-small cell lung carcinoma. They could be the important gene for regulation tumor cell proliferation and apoptosis , so could be used as a useful marker of a supplementary diagnostic and prognosis for patients with NSCLC. Examination of expression of two proteins may be helpful to judge biological behavior and prognosis of non-small cell lung carcinoma. |
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