Mannose 6-phosphate Modified Albumin Nanoparticles Drug Delivery System For Hepatic Stellate Cells Targeting

Neoglycoprotein (mannose 6-phosphate modified bovine serum albumin, M6P_n-BSA) were synthesized from mannose, followed by chemistry structure modification and linking-coupled with BSA. SF-M6P_n-BSA-NPs were prepared with sodium ferulate (SF) and neoglycoprotein by desolvation method. The shape of nanoparticles was observed by transmission electron microscope (TEM), while the mean diameter was 180nm and zeta potential was -23.9mv measured by laser light scattering technique. The mean entrapment efficiency and drug loading determined by ultrafiltration were 75.40% and 15.86% respectively. The concentration of sodium ferulate, and flow rate of ethanol showed direct correlation to the nanoparticles size while glutaraldehyde showed inverse correlation. All these factors have great importance to entrapment efficiency and drug loading. Delivery properties of SF-M6P_n-BSA-NP were consistent with the Weibull function in vitro. SF nanoparticles showed obviously targeting efficiency in liver. Double-immunostainings were performed on the liver slices in fibrotic rats after an intravenous of M6P-modified albumin nanoparticles which indicated that neoglycoprotein nanoparticles can be taken up by HSC.