Relationship Between The Expression Of Drug-resistant Genes In Candida Albicans Clinical Isolates And Fluconazole Resistance

Objective To explore the relationship between the expression of drug-resistant genes in Candida albicans clinical isolates and fluconazole resistance. Methods 2610 strains of Candida spp. were isolated and identificated by routine methods. Antifungal susceptibility testing was performed by using the Clinical and Laboratory Standards Institute(CLSI) reference broth microdilution M27-A. The RNA of fluconzole-resistant and fluconzole-susceptible Candida albicans isolates were extracted by the Kits, and cDNA were synthesized, then the expressions of CDR1, CDR2, MDR1, FLUl gene were observed by Real-time PCR methods. Results Higher expression of CDR2 and FLU1 gene were observed in the fluconzole-resistant Candida albicans isolates than that in the fluconzole-susceptile Candida albicans isolates, but the expression of CDR1 and MDR1 gene had no difference in statistics. Conclusions The formation of fluconzole resistance is very complicated, the mechanisms which have been identified so far alone are not sufficient, and most probably there are other putative mechanisms of resistance not yet described.