Treatment Of Experimental Endometriosis With Recombinant Human Interferon-alpha-2b In A Murine Model

Objective: To establish the model of rats endometriosis and determine the effect of human interferon(IFN)- α -2b on endometriosis in rats by detecting VEGF expression and microvessel density(MVD) in ectopic endometrum of model rats and compare the two treatments.Method: Sixty female SD rats' left uterine horn was resected in estrus,then implanted the self-endometrium fragments measuring 25mm~2 on the right peritoneal wall.After four weeks,thirty-eight rats with endometriosis were divided randomly into tree groups:groupA(n=15),groupB(n=15),control(n=8).The rats in groupA received 100,000u of IFN-α-2b by intraperitoneal(IP) deposit during the laparotomy.The rats in groupB were treated with three doses of IFN(100,000u every 48 hours)by SC injection .after the second laparotomy was performed.The control group was not given any treatment.Laparotomy was performed to measure thr size of each plant at various times (weeks 4, 6,and 8 after treatment),and resceted part of the implant.Tissues were fixed with formalin,paraffin-embedded and sectioned . Using SP immunohistochemical technique, we assess VEGF and MVD in ectopic endometrum of model rats .Result: The model of endometriosis in rats was established successfully , and there was 63.3 %(38/60) transplant to develop. The endometrial implants in the rats became cystic structures that were composed of endometrial gland and stroma. The macroscopic and histologic appearance was consistant with endometriosis.At the last laparotomy ,the mean size of the plants by animal were significantly smaller in the treated groups (A and B) than in the control group(p<0.05).The expression of VEGF and MVD in the ectopic endometrum of model rats were both decreased in the treated groups (A and B) than in thecontrol group(p<0.05). There were no differences between two treated groups (A and B) when we considered the mean volume , VEGF and MVD at the last laparotomy after the treatment of IFN (p>0.05) .Discussion: The model of endometriosis in rat established by implanting the self-endometrium is the convenient method to study and research the endometriosis . Two short regimens of human IFN- a -2b reduce the size of experimental endometriosis in rats.,perhaps by inhibiting the angiogenesis of ectopic endometrium, and provide a new therapy for endometriosis.