Epidemiological Features Of Warts In Patients From Shenyang, And Clinical Observation On The Effect Of Local Thermotherapy

IntroductionHuman Papillomaviruses ( HPV) are small closed circular double - stranded DNA virus, which infect cutaneous and mucocutaneous epithelia. A vast majority of these infections are either clinically unapparent or benign prolifera-tive conditions as warts or condylomas. Viral warts are one of the most common diseases of the skin, often occurring in children and young adults. There are many treatment options for viral warts such as liquid nitrogen, electrocautery, laser, herbal remedy, caustics acids and immune therapy etc. But no single modality can be considered the treatment of choice. Our research aims to learn a-bout the epidemiology characteristics of patients with warts from Shenyang and its surrounding areas by questionnairing in our clinic;extract HPV DNA from the wart scurf to learn about the molecular - epidemiology characteristics;treat the patients by using local thermotherapy to evaluate its efficacy.Subjects and methodsSubjects: all patients were from the dermatology clinic of No. 1 hospital of China Medical University who had been clinically diagnosed warts;the skin samples were wart scurf from the above patients who agreed to be scratched.Methods:1. ask the patients to fill the questionnaire forms which includes age sexcareer family history working environment lifestyle etc. to learn about the epidemiology characteristics of skin warts;2. Extract HPV DNA from the skin samples;firstly, wart scurf were digested by protease K ( concentration is 20mg/ml ) overnight, then extract HPV DNA by using the Universal Genomic DNA Extraction Kit Ver. 3. 0 DNA from TaKaRa company, run PCR with HPV skin type general primer HVP2/B5, fi-naly, detect the result by 1.5% sepharose electrophoresis, record the result with UV gel - imaging system;3. Use the regulable localized heating machine to treat the volunteers of warts patients.ResultsTotally 146 subjects from Shenyang and its surrounding areas were involved. There are no gender differences ( 74 males and 72 females ) , but age differences exist------teenagers are the high risk group, 74% patients are younger than 40 years. Verruca vulgaris is most common which is 47. 3% , seconded by verruca plantaris ( 40.4% ) , then verruca filiformis ( 10. 3% ) and verruca plana ( 2% ). The palms soles faces and necks are common sites of involvement ( totally 93. 8% of all ). In females, the feet are the most commonly involved ( 52. 8% ) , followed closely by the hands (29. 2% ). In males, the hands are the most common site (37. 8% ) , followed closely by the feet ( 28. 4% ).110 skin samples were detected, and there are 74 HPV DNA positive. The positive rate is 67. 3%. And the detected HPV DNA types included HPV - 1 2 3 4 10 41 57 etc.41 wart volunteers were treated by the local thermotherapy. But 5 patients were unavailable for follow - up ( follow - up visit less than 2 months ). Among the remaining 36 patients, 16 patients completely recovered, 8 patients'warts partly regressed and 12 patients'warts didnt change. The effective rate is 66. 7%.ConclusionsPreliminary data were obtained about the epidemiology and molecular - epidemiology characteristics of wart patients from Shenyang and its surrounding areas.Pilot treatment results showed that the local thermotherapy is effective and acceptable, especially for feet warts.