Correlation Analysis Of CD34-positive Cells And SDF-1 In Peripheral Blood Of Patients With Acute Myocardial Infarction

Background Acute myocardial infarct is becoming a worldwild health issue now.The myocardial remolding after myocardial infarction contribute to chronic heart failure,which lead to long run highly motality of those patients.Therefore ,it is the key to lower the motality and prevent myocardial remolding through increasing the myocardium number . Neovascularization is a new measure of treatment for ischemic heart disease and occlusive atherosclerosis.Endothelial progenitor cells (EPCs) circulate in adult peripheral blood (PB) contribute to neovascularization.However, little is known regarding whether EPCs and their putative precursor,CD34-positive mononuclear cells (MNC^cd34+), are mobilized and homing into PB in acute ischemic events in humans. Objective To investigate the Mobilization of CD34-positive mononuclear cells and the correlation between the mobilization and homing of CD34-positive cells and SDF -1 which in the peripheral blood of the patients with ST-segment elevation acute myocardial infarction (STEMI).Methods 40 patients with STEMI (<10 hours), 20 with stable angina, and 20 healthy control subjects were enrolled. The number of the cells was assessed by use of a flow cytometer,and the plasma level of SDF -1 and other 3 cytokines,vascular endothelial growth factor(VEGF),granulocyte colony-stimulating factor , (G-CSF), interleukin-6, (IL-6) , were measured using ELISA kits meantime. Data were subjected to one-way ANOVA followed by Q test for comparison between any 2 means. Use linear regression to analyze the relationship between the number of circulating CD34+ progenitor cells and plasma levels of Cytokines. Results: The number of CD34+ progenitor cells in peripheral blood was significantly higher in STEMI patients than in stable angina and healthy subjects,peaking at 7 days after MI. The plasma level of SDF-1 . VEGF elvated meantime. Simple regression analysis revealed that the number of circulating CD34+ progenitor cells positively correlated with the plasma levels of SDF -1 (R=0.53, p<0.001). In logistic regression,only the significant increase of SDF-1 level (defined as SDF-1 concentration 1000 pg/mL at 24 hours after admission)was an independent predictor of significant increase(100 cells/uL [median]) of CD34+ cell numbers.Conclusion The number of MNC^CD34+ in the peripheral blood of patients with myocardial infarction is higher than the healthy people. SDF -1 is crucial to mobilization, homing, and survival of CD34+ progenitor cells in myocardial infarction.