| Objective To study the change of the C9 and MCP-1 deposition in cerebral ischemia-reperfusion tissues of rat. Defibrase was administered to observe its effect on complement system and corresponding inflammatory injury. Methods Rat models of the focal cerebral ischemia-reperfusion were used and defibrase was administered in one group, then followed by immunohistochemical stain to detect C9, MCP-1 deposition in each group. Results 1) At 3 hours after ischemia , C9 appeared in brain around the ischemia, peaked at 24 hours; The MCP-1 raised at 6 hours , peaked at 24 hours after chemia-reperfusion and maintained high level. 2) The expression of C9 and MCP-1 around the ischemia reduced significantly after given defibrase (P<0.01 or P<0.05) . Conclusions Complement and inflammatory reaction participated in cerebral ischemia-reperfusion injury. Defibrase could reduce C9, MCP-1 position in ischemia tissues, which led to decreased leukocytes infiltration and less tissue injury.
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