The Intervening Effect Of Resveratrol On Renal Lesion In Diabetic Rats

Objective To explore the effects of resveratrol on oxidative stress status and the intervene effect on nephritic insult in diabetic rats.Methods 55 male Wistar rats (3 months old) were fed by fat-enriched and high-energy diet. Diabetes rats were induced by the intravenous administration of streptozotocin (STZ) at a dose of 30mg/kg body wt when the rats weighted about 400g (n=44) . Normal control rats were received same volume menstruum alone (n=9) . The diabetic rats were randomly divided into diabetes mellitus control rats (Group DC);low-dose resveratrol treated (10mg·kg^-1-d^-1 ) group (Group RL);middle-dose resveratrol treated (20mg·kg^-1·d^-1) group (Group RM) and high-dose resveratrol treated (40mg·kg^-1·d^-1) group (Group RH). All rats were sacrificed at the end of 10th week after models were established. The levels of blood glucose, urine protein, glomerular basement membrance thickness (GBMT), profile of kidney hypertrophy, serum and renal total antioxidative capacity (TAC), malondialdehyde (MDA), thiol group (SH) and nitric oxide (NO), urea nitrogen (BUN), creatinine (Cr) were measured and analysed. The renal pathologic changes were observed by light microcopy and electron microscopy. The expression of nuclear factor-κB (NF-kB) and monocytechenoattractant protein-1 (MCP-1) in the renal was examined by immunohistochemical methods.Results 1. The level of blood glucose in diabetic rats were significantly higher than that in the group NC (P <0. 01) , the groups that treated with resveratrol was nearly same as that in diabetic rats's (p>0.05). 2. The levels of serum and renal cortex TAC, SH, NO were significantly decreased in diabetic rats as compared with normal control animals (p<0.01), the contents of MDA were significantly higher than that in the group NC;but in diabetic rats treated with low-dose resveratrol, those targets were significantly improved (p<0.05,p<0.01). 3. The expression of NF-kB and MCP-1 were significantly increased in group DC as compared with group NC (p<0.01) , it can be down regulation by low-dose resveratrol treated(p<0.01). 4. Renal pathologic changes were also improved in resveratrol treated groups as compared with group DC. It cound be found that the GBMT and MGV were obviously thicker and bigger in group DC than that of group RL, RM and NC(p<0.05, p0.01). 5. The urine protein and profile of kidney hypertrophy and serum BUN, Cr in group DC were allsignificantly higher than that in group NC, treatment with resveratrol led to an improvement in those targets(p<0.05,/?<0.01), there was an emphasis on low-dose resveratrol.Conclusion 1. There were the oxidant-antioxidant unbalance in diabetic rats;oxidative stress may play an essential role in the progress of diabetic nephropathy. 2. Resveratrol can effectively inhibits oxidative stress and down regulation of the expression of NF-kB and MCP-1 in the kidney notwithstanding hyperglycemia in diabetic rats. It can improve the renal pathologic changes. Administration of resveratrol to diabetic rats can ameliorate the contents of urine protein > serm BUN and Cr, it can protect nephritic insult in diabetic rats.