Effects Of Ganglioside GM₁ On The Regeneration Of Rabbit Injury Facial Nerve

Objective: (1)To study the effect of ganglioside GM1 on the regeneration of facial nerve. (2)To offer theorical and experimental basement for using GM1 in regeneration of facial nerve at early clinical stage. Methods: Eighteen healthy adult New Zealand rabbits were divided into two groups randomly experimental group(GM1) and control group(normal sodium), To established animal models of facial nerve injury. 2.0ul(10ug) GM1 was dropped in place of anastomosis for experimental group, and 2.0ul(10ug) normal sodium was dropped in place of anastomosis for control group. Nerve conduction velocity and histological changes of facial nerve were observed in 2, 4 and 8 weeks after surgery separately. Results: there were statistical difference of nerve conduction .velocity between the two groups, in which the experimental group was superior to that of the control group after 2 and 4 weeks of surgery (P<0.05) .No statistical difference was found in nerve conduction velocity between the experimental and control group after 8 weeks of surgery (P>0.05) . The nerve regeneration was observed using light microscope, in which experimental group was superior to that of the control group after 2 and 4 weeks of surgery. No difference was found in nerve regeneration between the two group after 8 weeks of surgery. Conclusion: Proper dosage of ganglioside GM1 are effective for regeneration of facial nerve at the early stage of injury.